Evidence map›Paper›PMID 41669890›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Testicular mRNA-LNP Delivery: A Novel Therapy for Genetic Spermatogenic Disorders.

Chenwang Zhang, Nan Liang, Wenbo Li, Shuai Xu, Peng Li, Wanze Ni, Na Li, Sha Han, Ningjing Ou, Haowei Bai and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Chenwang ZhangDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.ORCID https://orcid.org/0000-0001-9186-7125
Nan LiangDepartment of Human Cell Biology and Genetics, SUSTech Homeostatic Medicine Institute, Joint Laboratory of Guangdong & Hong Kong Universities For Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Wenbo LiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shuai XuDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Peng LiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Wanze NiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Na LiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Sha HanDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Ningjing OuDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Haowei BaiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yuxiang ZhangDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Furong BaiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yifan SunDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Dewei QianDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xinjie BuDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Erlei ZhiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Ruhui TianDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yuhua HuangDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jingpeng ZhaoDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Fujun ZhaoDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Hao ChenDepartment of Human Cell Biology and Genetics, SUSTech Homeostatic Medicine Institute, Joint Laboratory of Guangdong & Hong Kong Universities For Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Zheng LiDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Chencheng YaoDepartment of Andrology, the Center for Men's Health, Urologic Medical Center, Reproductive Medicine Center, Shanghai Key Laboratory of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.ORCID https://orcid.org/0000-0002-8820-7895

Funding

Emerging advanced technology joint research project of SHDC 20231120115406001Emerging advanced technology joint research project of SHDC 20240812083401001Emerging advanced technology joint research project of SHDC SHDC12023121National Key R&D Program of China 2022YFC2702700National Key R&D Program of China 2022YFC2703004National Natural Science Foundation of China 82171590National Natural Science Foundation of China 82371607National Natural Science Foundation of China 82371616Pearl River Recruitment Program of Talents 2021QN02Y122Science, Technology and Innovation Commission of Shenzhen Municipality 20231120115406001,20240812083401001
6 · The paper itself

Abstract

Uniform testicular maturation arrest is a severe form of male infertility characterized by the presence of germ cells that do not complete spermatogenic development. It is usually caused by meiotic arrest with genetic variants and is difficult to treat via drugs or surgery. mRNA-lipid nanoparticle (LNP) delivery is a promising therapeutic option for maturation arrest with monogenic variants via protein replacement therapy. Herein, a spermatocytes-tropic LNP (Pool1-LNP3) was identified via a library of 30 ionizable lipids screening. And in vivo delivery of this novel LNP composition using rete testis microinjection was shown to be high spermatocytes targeting with high transfection efficiency. Thereafter, it was revealed that in vivo delivery of Pool1-LNP3 encapsulating Msh5 mRNA could promote crossover formation and restore spermatogenesis in Msh5

Indexed as

Infertility, MaleNanoparticlesRNA, MessengerSpermatogenesisTestisAnimalsDisease Models, AnimalLiposomesMaleMiceLipid NanoparticlesLiposomesRNA, Messengergenetic defectsmale infertilitymRNA‐LNP deliveryrestoration of spermatogenesisspermatogenic disorders

Identifiers

PMID41669890
PMCPMC13088321

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.