Greenness Evaluation of Piracetam Detection Through Spectrofluorimetric Method With Shilajit-Derived Metal Oxide Nanosensors.

Piracetam (PRM) is a nootropic commonly used to improve cognitive function, memory, and learning ability. This method introduces a new spectrofluorimetric strategy for the identification of PRM, using metal oxide nanomaterials from Shilajit extract in a micellar medium. The technique is based on a unique fluorescent platform of aluminum oxide and nickel oxide nanoparticles (NPs) combined with sodium dodecyl sulfate (SDS). The metal oxide NPs were prepared by an environmentally friendly synthesis approach, using Shilajit extract as a dual-function agent for reduction and stabilization. Their morphology, size, and structural properties were comprehensively analyzed using a range of spectroscopic and microscopic methods. The innovative technique utilizes the unique fluorescence properties of alumina and NiO NPs in the presence of SDS to detect PRM with remarkable sensitivity and selectivity. This method enables high-precision measurements over a wide calibration range of 0.5-10 and 0.2-14 μg/mL for the two metal oxides, respectively. With PRM recoveries of 99.07% ± 0.65% and 99.60% ± 0.37%, the method has excellent accuracy and reliability. Medium precision was used to ensure that the method meets stringent precision standards. In addition, the environmentally friendly approach of using Shilajit extract for the sustainable synthesis of metal oxides reduces the impact on the environment while maintaining excellent analytical performance, as confirmed by an environmental impact assessment.