Evidence map›Paper›PMID 41666336›Full record

ReviewACS synthetic biology2026

Cyanobacterial Biofertilizer Production by Guanidine-Producing Enzymes.

Hakyung Lee, Jacob Sebesta, Eric Schaedig, Chao Wu, Himadri B Pakrasi, Jianping Yu

Abstract readReview
In one paragraph

Review in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hakyung LeeBioscience Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.ORCID 0000-0002-1064-4227
Jacob SebestaBioscience Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.
Eric SchaedigBioscience Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.ORCID 0000-0002-9310-4946
Chao WuBioscience Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.ORCID 0000-0003-3642-4609
Himadri B PakrasiWashington University, Saint Louis, Missouri 63130, United States.ORCID 0000-0001-8240-2123
Jianping YuBioscience Center, National Laboratory of the Rockies, Golden, Colorado 80401, United States.ORCID 0000-0003-0466-3197

Funding

National Laboratory of the Rockies DE-AC36-08GO28308U.S. Department of Energy (DOE) DE-AC36-08GO28308
6 · The paper itself

Abstract

Cyanobacterial production of a biofertilizer shows promise as an environmentally benign alternative to conventional nitrogen fertilizers, reducing environmental and energy burdens through light-driven nitrogen and carbon fixation. One route to realizing the potential for a nitrogen-rich, slow-releasing biofertilizer involves the genetic engineering of cyanobacteria to produce guanidine. Recent advances have demonstrated enzymatic guanidine production in cyanobacteria, but an understanding of cyanobacterial guanidine metabolism is still limited. This Perspective highlights strategies and opportunities for cyanobacterial guanidine production in a Design-Build-Test-Learn cycle. Exploring new guanidine-producing enzymes via phylogenetics could expand candidate enzymes, while understanding the metabolism of substrates can identify constraints and opportunities in substrate utilization. Additionally, guanidine sensing and export are crucial areas of study to enable continuous fertilizer production and stable nitrogen flux. These strategies will guide the development of advanced nitrogen biofertilizer strategies for the agricultural sector.

Indexed as

CyanobacteriaFertilizersGuanidineBacterial ProteinsNitrogenNitrogen FixationBacterial ProteinsFertilizersGuanidineNitrogen

Identifiers

PMID41666336
PMCPMC12930504

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.