ArticleAnalytical and bioanalytical chemistry2026
Derivatization agents for LC-MS analysis of amino acids: effects of core structure and functional groups.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Standard Reference Material (SRM®) 1950 metabolites in frozen human plasma-use, impact, lessons learned, and future perspectives.Analytical and bioanalytical chemistry · 2026Review
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3 authors.
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Abstract
The analysis of amino acids in biological samples is challenged by their high polarity, low ionization efficiency, and lack of chromophores, which limit detection by LC-UV or LC-MS. Derivatization is widely used to improve retention, sensitivity, and detection. In this study, derivatization agents based on pyridine, quinoline, and isoquinoline positional isomers scaffolds with COCl, SO₂Cl, and NHS ester reactive groups were systematically evaluated using deuterium-labeled amino acids to assess stability, derivatization efficiency, chromatographic behavior, and MS response. NHS ester-based agents were found to exhibit superior stability, maintaining activity for over 1 year, whereas carbonyl chlorides and sulfonyl chloride-based agents were highly reactive but less stable. NHS ester of isoquinoline-6-carboxylic acid (6-CiQ-NHS) was identified as the most effective agent, combining rapid derivatization, strong MS signal, and stability. LC-MS analysis demonstrated excellent linearity (R
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