ArticleApplied and environmental microbiology2026
An RTCA-based assay as an innovative approach for thermal inactivation studies of hepatitis A virus.
Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Hepatitis A virus (HAV) is responsible for acute viral hepatitis worldwide, mainly transmitted via the fecal-oral route either through direct contact or by consuming contaminated food. Thermal inactivation is widely applied in the food industry to inactivate viruses and ensure the safety of food products. The effectiveness of inactivation treatments depends on the initial viral load and the sensitivity of the method quantifying infectious viruses. This study aimed to assess the real-time cell analysis (RTCA) assay in the framework of heat inactivation studies, compare its suitability to the traditional PFU assay and molecular-based methods (RT-qPCR, integrity-RT-qPCR), and model thermal inactivation kinetics. Our results showed that the RTCA assay is a suitable method for quantifying the decrease in infectious HAV as the time-temperature scale increases. No more infectious virus was detected after treatment at 37°C, 50°C, 65°C, 72°C, and 80°C, lasting 9 days, 24 h, 10 min, 5 min, and 2 min, respectively. The Geeraerd model was identified as the best fit to describe HAV thermal inactivation kinetics. The
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.