Evidence map›Paper›PMID 41663553›Full record

ArticleScientific reports2026

Experimental and molecular docking analyses of antibacterial activity in moroccan Rosmarinus officinalis essential oil.

Youssef Lahlou, Soukaina Elorchi, Mohamed Dakir, Mohammed Salah, Nirmine Ezzouine, Malika Belfaiza, Kacem Makroum, Abdellah Maissour

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Frontiers in bioinformatics · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Youssef LahlouLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco.
Soukaina ElorchiMolecular Modeling and Spectroscopy Research Team, Department of Chemistry, Faculty of Sciences, Chouaïb Doukkali University, El Jadida, Morocco.
Mohamed DakirLaboratory of Organic Chemistry, Materials, Electrochemistry, and Environment, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, B.P.2693, Maarif, 20000, Morocco.
Mohammed SalahMolecular Modeling and Spectroscopy Research Team, Department of Chemistry, Faculty of Sciences, Chouaïb Doukkali University, El Jadida, Morocco.
Nirmine EzzouineLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco.
Malika BelfaizaLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco.
Kacem MakroumLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco.
Abdellah MaissourLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, URL-CNRST n°10, Faculty of Sciences, University Chouaib Doukkali, El Jadida, Morocco. maissour.a@ucd.ac.ma.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimicrobial resistance motivates antibacterial agents with multi-target mechanisms. We evaluated Moroccan rosemary essential oil (ROEO) against four pathogens (Escherichia coli, Citrobacter freundii, Staphylococcus aureus, Enterococcus faecalis) by disk diffusion and broth microdilution. ROEO inhibited all strains (zones 11.3-21.0 mm); activity was bactericidal for E. faecalis (MBC/MIC = 2.0) and bacteriostatic for others (MBC/MIC > 4). Bioassay-guided silica chromatography, using E. faecalis as the pre-specified indicator, localised activity to a polar fraction (F8; 18.5% of the oil; 14.0 ± 1.71 mm). GC-MS showed F8 was enriched in oxygenated monoterpenes (97.45%): myrtenol, verbenone, p-cymen-8-ol, γ-terpinen-7-al, carvone, β-thujone. Docking (AutoDock Vina) predicted binding of major constituents to essential enzymes (tyrosyl-tRNA synthetase, L-methionine γ-lyase, DNA gyrase B, and NAD⁺-dependent DNA ligase); native-ligand redocking reproduced crystallographic poses (RMSD ≤ 2.0 Å). In silico ADMET supported drug-like properties with high intestinal absorption and class-typical CNS-penetration and skin-sensitisation alerts. ROEO's antibacterial profile is underpinned by an oxygenated-monoterpene fraction and yields testable hypotheses for enzyme validation, synergy studies, and in vivo efficacy and safety.

Indexed as

Anti-Bacterial AgentsMolecular Docking SimulationOils, VolatileRosmarinusMicrobial Sensitivity TestsMonoterpenesMoroccoAnti-Bacterial AgentsMonoterpenesOils, VolatileAntibacterial activityBioassay-guided fractionationEnterococcus faecalisGC–MSMolecular dockingRosmarinus officinalis

Identifiers

PMID41663553
PMCPMC12953884

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.