ArticleScientific data2026
Chromosome-level genome assembly of the medicinal plant Ophiorrhiza japonica Blume.
Article in Scientific data, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ophiorrhiza japonica, a medicinal plant of Rubiaceae, has been selected as a model plant for the study of MIA biosynthesis and regulation, as well as a sustainable source of camptothecin. Here, we performed an assembly and annotation of O. japonica genome. To achieve this, we employed a range of advanced techniques, including flow cytometry, PacBio HiFi sequencing, ONT RNA-sequencing and Hi-C technology. This approach enabled us to construct a high quality, chromosome-level genome of O. japonica. The assembled O. japonica genome spanned 549.81 Mb with a contig N50 size of 43 Mb and a scaffold N50 size of 46.45 Mb. The 24 contigs, representing 99.42% of the total assembled genome, were anchored to 11 chromosomes using Hi-C scaffolding. A total of 313.49 Mb of repeat sequences were identified and 28,182 protein-coding genes were predicted. The findings of this study provide invaluable genomic resources that will facilitate a deeper understanding of species evolution and enable the investigation of a range of crucial traits.
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