Evidence map›Paper›PMID 41663215›Full record

ArticleEmerging infectious diseases2026

Multiplex PCR to Differentiate Monkeypox Virus Clades.

Christopher T Williams, Alessandra Romero-Ramirez, Adeleye Adesola Semiu, Samuel Oluwafunmbi Ifabumuyi, Caitlin Greenland-Bews, Susan Gould, Dominic Wooding, Collette Allen, Anushri Somasundaran, Nicodemus Nnabuike Mkpuma and 17 more

Abstract read
In one paragraph

Article in Emerging infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Christopher T Williams
Alessandra Romero-Ramirez
Adeleye Adesola Semiu
Samuel Oluwafunmbi Ifabumuyi
Caitlin Greenland-Bews
Susan Gould
Dominic Wooding
Collette Allen
Anushri Somasundaran
Nicodemus Nnabuike Mkpuma
Dorcas Gado
Jolly Amoche Adole
Abdulakeem Eniola Amoo
Abisola Ajoke Adeyemi
Laure Bosquillon de Jarcy
Christine Goffinet
Jake Dunning
Malcolm G Semple
Esto Bahizire
Afolabi Akinpelu
Thomas E Fletcher
Ana I Cubas-Atienzar
Cristina Leggio
Adeyinka Adedeji
Adesuyi A Omoare
Thomas Edwards
International Severe Acute Respiratory and Emerging Infection Consortium Investigators

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

Indexed as

Monkeypox virusMpox, MonkeypoxMultiplex Polymerase Chain ReactionHumansNigeriaPhylogenySensitivity and SpecificityUnited Kingdomdiagnosticsmonkeypox virusmpoxMPXVNigeriaPCRUnited Kingdomviruseszoonoses

Identifiers

PMID41663215
PMCPMC12928233

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.