ArticleJournal of fluorescence2026
Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.
Article in Journal of fluorescence, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
As a remarkable model protein for studying excitation energy transfer (EET), Cryptophyte phycocyanin 645 (Cr-PC645) is not only important in the frontier research field on photosynthesis but also, like other kinds of phycocyanin, valuable in food and cosmetic industries as a kind of natural blue pigment protein. Here, we report a convenient preparation method for the purification of Cr-PC645 from cryptophytic algae, Chroomonas placoidea, mainly using hydrophobic interaction chromatography, and a study of EET characterization based on it. The final recovery of Cr-PC645 is 58.49% and the highest purity of final samples reaches 7.27 (A645/A280) in the obtained products. In addition to high purity, the properties of unchanged intact optical activity of purified Cr-PC645 are proved by comprehensively analyzing absorption, fluorescence, circular dichroism, and ultrafast time-resolved spectra. Furthermore, three time components of 6, 59, and 1021 ps are confirmed by 77 K time-resolved fluorescence spectroscopy experiments, which can be assigned to the time of energy transfer process between specific phycocyanobilins (PCBs), suggesting that the excitation energy is transferred from PCB-158 to PCB-82 in the same β subunit and localized the PCB-82 chromophores between different β subunits, then transferred to the downstream acceptors. In view of all these results, this optimized method is efficient and will be conducive to multi-field applications in the future.
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