Evidence map›Paper›PMID 41661346›Full record

ArticleBreast cancer research and treatment2026

Disease- and chemotherapy-associated salivary microbiome changes in breast cancer patients.

Elżbieta Kaja, Joanna Grupińska, Magdalena Budzyń, Joanna Ciomborowska-Basheer, Anita Szwed, Izabela Makałowska, Zacharias Papadovasilakis, Dorota Formanowicz

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Article in Breast cancer research and treatment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Elżbieta KajaChair and Department of Medical Chemistry and Laboratory Medicine, Poznan University of Medical Sciences, Poznań, Poland. ekaja@ump.edu.pl.ORCID http://orcid.org/0000-0003-1277-6140
Joanna GrupińskaChair and Department of Medical Chemistry and Laboratory Medicine, Poznan University of Medical Sciences, Poznań, Poland.ORCID http://orcid.org/0000-0002-1814-1771
Magdalena BudzyńChair and Department of Medical Chemistry and Laboratory Medicine, Poznan University of Medical Sciences, Poznań, Poland.ORCID http://orcid.org/0000-0003-2296-3599
Joanna Ciomborowska-BasheerLaboratory of Nature Education and Conservation, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, Poznań, Poland.ORCID http://orcid.org/0000-0003-1166-3777
Anita SzwedInstitute of Human Biology and Evolution, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, Poznań, Poland.ORCID http://orcid.org/0000-0001-7516-5019
Izabela MakałowskaInstitute of Human Biology and Evolution, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, Poznań, Poland.ORCID http://orcid.org/0000-0002-8144-5671
Zacharias PapadovasilakisInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Greece.ORCID http://orcid.org/0000-0001-8255-5090
Dorota FormanowiczChair and Department of Medical Chemistry and Laboratory Medicine, Poznan University of Medical Sciences, Poznań, Poland.ORCID http://orcid.org/0000-0001-6691-3863

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe microbiome of the saliva can be influenced by various factors, including systemic diseases and chemotherapy. Oral dysbiosis manifests as altered bacterial composition and abundance, which often correlates with increased local and systemic inflammation. The aim of the study was to investigate the dysbiosis in the saliva of breast cancer (BC) patients before and during neoadjuvant chemotherapy (NAC).

methodsSaliva samples were collected from 50 breast cancer patients at three timepoints (before, during, and after NAC). Saliva from 10 healthy women was used as control samples. Full-length gene 16S rRNA sequencing and analysis were performed using the Microbiome Analyst platform, R and JADBIO AutomatedML platform to compare the abundances of bacterial taxa.

resultsAlpha and beta diversity measures differed between breast cancer patients and healthy controls. In addition, eight bacterial genera differed significantly between breast cancer patients and controls, including Porphyromonas, Campylobacter, Oribacterium, Veillonella, and Alloprevotella. Longitudinal analysis revealed significant decrease of bacterial diversity in the course of neoadjuvant chemotherapy as well as significant change in the prevalence of a few low-abundant genera.

conclusionsThe obtained results confirm BC-related and NAC-related dysbiosis in saliva, which emphasizes the potential of saliva as a diagnostic and prognostic tool in patients with breast cancer.

Indexed as

Breast NeoplasmsDysbiosisMicrobiotaSalivaAdultAgedBacteriaCase-Control StudiesFemaleHumansMiddle AgedNeoadjuvant TherapyRNA, Ribosomal, 16SRNA, Ribosomal, 16S16S rRNA sequencingBreast cancerNanopore sequencingNeoadjuvant chemotherapyOral microbiome

Identifiers

PMID41661346

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.