ArticlePlanta2026
Overexpression of Chlorella ellipsoidea DGAT1 gene in soybean improves oil content and influences lipid metabolism.
Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
MAIN
conclusionOverexpression of Chlorella ellipsoidea DGAT1 in soybean increases oil content and changes the components of fatty acids in seeds, resulting a great influence on lipid metabolism. High oil transgenic soybean germplasm is important for expanding soybean breeding resources. Here, CeDGAT1, a gene from Chlorella ellipsoidea encoded a key rate-limiting enzyme in triacylglycerol synthesis, was introduced into soybeans by Agrobacterium-mediated soybean cotyledon node method, and we have obtained an overexpression line CeDGAT1-OE#7 based on soybean cultivar Dengke12. Firstly, qRT-PCR analyses displayed that CeDGAT1 was expressed in all the tissues of the transgenic line CeDGAT1-OE#7. And the total oil content was significantly higher by an average of 2.27% in seeds compared with the wild type. The analyses for the components of fatty acids showed that palmitic acid (16:0), stearic acids (18:0), and oleic acid (18:1) increased significantly by an average percentage of 1.34%, 0.62%, and 5.91%, respectively, while the contents of linoleic acid (18:2) and linolenic acids (18:3) were significantly lower than that of the WT. Furthermore, metabolomics analyses were conducted by using developing seeds of three stages, and a total of 1467, 1461, and 1078 significantly differentially expressed metabolites (DEMs) were separately identified. Two lipid-related pathways, linoleic acid metabolism and alpha-linolenic acid metabolism, were identified using KEGG pathway analysis of these DEMs, and seven metabolites in the linoleic acid metabolism pathway were down-regulated simultaneously, suggesting that these metabolites might be crucial in the response for overexpression of CeDGAT1. Together, our study has developed a high-oil transgenic soybean germplasm as a future breeding resource and those findings obtained from this research have broadened our understanding of the molecular mechanism underlying oil accumulation and fatty acid metabolism.
Indexed as
Identifiers
41661319What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.