ArticleLung2026
Repetitive Exposure to Nicotine-Free Vaping Impairs Airway Defences and Delays Recovery.
Article in Lung, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 1 synthesis or guideline pooled it.
- Association of active and passive smoking with adolescent asthma: a systematic review and meta-analysis.BMC public health · 2026Pooled it
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeE-cigarette use, or vaping, is increasing in prevalence, especially among adolescents. There is a paucity of information on the impact of e-cigarette aerosols on lung health, particularly to the airway protective components. In this study, disruptions to airway protective functions in response to increasing repetitive exposure to nicotine-free e-cigarette aerosols were examined. The recovery capability of the airway defence was also investigated.
methodsCultured primary nasal airway cells from five healthy individuals were exposed to repetitive exposures to e-cigarette aerosols. Cilia function was analysed via high-speed video microscopy, while epithelial barrier integrity was assessed through trans-epithelial electrical resistance measurements and immunofluorescence. Cytokine secretion was assessed using multiplex screening.
resultsSignificant impairment in cilia function and epithelial barrier integrity was observed after 24 or more vaping exposures. Cilia beating frequency declined by 18.6% (6.78 ± 0.23 Hz) after 24 sessions and by 53.0% (3.91 ± 0.27 Hz) after 60 sessions (p < 0.01). Trans-epithelial electrical resistance values dropped by 45.6% (315.37 ± 13.38 Ωcm²) after 24 sessions and by 77.4% (131.29 ± 6.84 Ωcm²) after 60 sessions (p < 0.01). Nicotine-free aerosols disrupted Occludin-1 and ZO-1 expression but not E-cadherin. Cytokine analysis showed increased secretion of IL-1ra, IL-6, FGF-basic, IFN-γ, and VEGF following 36 vaping sessions. Recovery experiments indicated cilia function normalised within 48 h, while epithelial barrier required up to 72 h.
conclusionRepetitive vaping progressively impairs airway protective functions in vitro. While cilia function and epithelial integrity recover in the short term, the delay may increase susceptibility to infections.
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