Evidence map›Paper›PMID 41660905›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2026

Clausenidin from Clausena excavata Burm. causes Apoptosis of Liver Cancer in Mice.

Peter M Waziri, Richard Auta, Mustapha U Imam, Ben A Chindo, Zakari Ladan, Zainab Kasim Mohammed, Samson Wayah, Jaafar Mohammed Sani, Godwin I Ayuba, Oluwafemi T Ige and 2 more

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Article in Asian Pacific journal of cancer prevention : APJCP, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Peter M WaziriDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.ORCID 0000-0003-4843-584X
Richard AutaDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.
Mustapha U ImamDepartment of Medical Biochemistry, Usmanu Danfodio University, Sokoto, Nigeria.
Ben A ChindoDepartment of Pharmacology, Kaduna State University, Kaduna, Nigeria.
Zakari LadanDepartment of Chemistry, Kaduna State University, Kaduna, Nigeria.
Zainab Kasim MohammedDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.
Samson WayahDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.
Jaafar Mohammed SaniDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.
Godwin I AyubaDepartment of Anatomic Pathology and Forensic Medicine, Kaduna State University, Kaduna, Nigeria.
Oluwafemi T IgeDepartment of Medical Microbiology and Parasitology, Kaduna State University, Kaduna, Nigeria.
Daniel TyoapineDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.
Abel AgbajiDepartment of Biochemistry, Kaduna State University, Kaduna, Nigeria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundClausenidin is a pyranocoumarin that was isolated from the roots of Clausena excavata Burm. Previously, the pure clausenidin crystals were successfully used to treat HepG2 cells (liver cancer) in vitro; however, no in vivo study had been conducted to support these findings. Therefore, the current study was designed to investigate the in vivo anti-liver cancer effect of pure clausenidin in hepatocellular carcinoma-induced BALB/c mice.

methodDNA fragmentation, caspases, and histological assays were conducted to evaluate the cytotoxic effect of the pure clausenidin, while real-time qPCR was performed to monitor the expression of apoptosis-inducing genes in the clausenidin-treated mice.

resultClausenidin significantly (p<0.05) decreased the liver damage-induced levels of alanine and aspartate aminotransferases and also caused fragmentation of the genomic DNA of the tumors. This was followed by a significant increase (p<0.05) in the expression of caspases 3, 8 and 9 proteins in the clausenidin-treated mice. In addition, clausenidin upregulated the expression of  pro-apoptotic genes associated with the extrinsic and intrinsic pathways. However, it was observed that clausenidin may have inhibited angiogenesis by downregulating the expression of the VEGF gene in the treated mice.

conclusionTherefore, clausenidin can be potentially used as an anti-liver cancer agent.

Indexed as

ApoptosisCarcinoma, HepatocellularClausenaLiver NeoplasmsAnimalsCell ProliferationHumansMaleMiceMice, Inbred BALB CApoptosisClausenidinHepatocellular carcinomaMDM2P-53

Identifiers

PMID41660905
PMCPMC13495528

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