Evidence map›Paper›PMID 41660674›Full record

ArticleFood chemistry. Molecular sciences2026

Performance difference of enrofloxacin monoclonal antibody and nanobody elicited from the same immunogen and the underlying molecular mechanism.

Yujie Chen, Zhaoxiang Wang, Qingqing He, Haiyan Xing, Simin Shen, Rui Feng, Yixuan Wu, Baomin Wang, Qing X Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry. Molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yujie ChenCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Zhaoxiang WangState Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Qingqing HeCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Haiyan XingCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Simin ShenState Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Rui FengCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Yixuan WuCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Baomin WangCollege of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China.
Qing X LiDepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enrofloxacin (ENR) is a widely used fluoroquinolone antibiotic in animal husbandry, aquaculture, and humans. Here, a monoclonal antibody (mAb 2D3) and a nanobody (Nb22) against ENR were generated with the same immunogen. Nb22 had the nanobody common property of greater stability in harsh conditions, but its assay sensitivity was approximately 30-fold lower than that of mAb 2D3. Nb22 showed better selectivity, which the cross-reactivity to each of ENR analogs was less than or equal to that of mAb 2D3. The VH and VL gene sequences were amplified from the hybridoma cell line 2D3. Molecular docking revealed that mAb 2D3 had stronger hydrogen bonds and formed a flat and wide binding pocket to accommodate other analogs of ENR. The average recoveries of ENR from milk, milk powder, egg and fish determined by mAb 2D3 and Nb22 based ic-ELISAs ranged from 77.7% to 119% and 88.2% to 116%, respectively. This study confirmed that the direct application of nanobody in immunoassay is no better than the conventional monoclonal antibody. Improving the sensitivity of nanobody is an essential prerequisite for taking advantage of its stability and specificity.

Indexed as

EnrofloxacinImmunoassayMolecular dockingMonoclonal antibodyNanobody

Identifiers

PMID41660674
PMCPMC12874607

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.