Epithelial Cell-Specific Prognostic Signature (FTH1, RIT1, WASL, NDRG2, KIFC3) Stratifies Cervical Cancer Patients and Correlates With Immune Infiltration.

Background: Cervical cancer (CC) remains one of the leading female malignancies. Epithelial cells (EpCs), primarily derived from the cervical squamous and glandular epithelium, are targeted by human papillomavirus to drive CC. Herein, we aimed to develop an EpC-specific risk model to improve clinical outcomes and unravel tumor immune microenvironment alterations in CC. Methods: scRNA-seq data from GSE208653 were processed using Seurat (including SCTransform for normalization and Harmony for batch correction). EpC heterogeneity was analyzed via subclustering, pseudotime trajectory analysis with monocle2, and cell-cell communication inference with CellChat. The hdWGCNA package identified EpC-specific coexpression modules. Prognostic genes were screened by univariate Cox and LASSO regression, and a Riskscore model was built using multivariate Cox regression. Immune infiltration was assessed by ssGSEA, MCPCounter, and ESTIMATE algorithms. Drug sensitivity correlation was analyzed using pRRophetic. In vitro functional assays validated key gene roles in CC cells. Results: Forty thousand four hundred fifty-seven cells were annotated into eight cell populations with a lower percentage of EpCs. Thereafter, EpCs were categorized into three subclusters with specifically highly expressed genes in peculiar biological pathways and with distinct trajectories of fate. A strong cell-cell communication network was observed, particularly involving Ep C3 and immune cells, via ligand-receptor pairs such as LGALS9-CD44 and HBEGF-EGFR. The hdWGCNA analysis revealed Ep C3-specific gene modules, from which a five-gene prognostic signature ( Conclusion: A proposed EpC-specific gene signature for CC may be applicable to support clinical decision-making.