ArticleJournal of conservative dentistry and endodontics2026
Wharton's jelly mesenchymal stem cell-derived exosomes promote odontogenic differentiation of dental pulp stem cells: A cell-free strategy for regenerative endodontics.
Article in Journal of conservative dentistry and endodontics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Noncoding RNA networks in endodontic regeneration: Current evidence and future directions.Journal of conservative dentistry and endodontics · 2026Review
- Exosome-loaded human amniotic membrane hydrogel modulates regenerative responses of human dental pulp stem cells.Journal of conservative dentistry and endodontics · 2026Article
- Comparative Analysis of Different Origin-Derived Exosomes for Future Regenerative Endodontic Therapies: A Systematic Review.Clinical, cosmetic and investigational dentistry · 2026Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Regenerative endodontics aims to restore the dentin-pulp complex through biologically based strategies shifting from traditional cells to cell-free approaches, particularly utilizing exosomes which are nanoscale extracellular vesicles that relevant for dental pulp regeneration. Wharton's Jelly MSC derived exosomes (WJ MSC Exo) offer an abundant, ethically acceptable, and biologically potent alternative with promising odontogenic and angiogenic properties. Aim: The aim of the study is to evaluate the regenerative potential of exosomes, particularly those from WJ-MSCs, as a safe and translational tool for dentin-pulp complex regeneration that focusing on metrics of cell viability, alkaline phosphatase (ALP) activity as an early differentiation marker, and late-stage mineralization. Subjects and Methods: Exosomes isolated from umbilical cord-derived WJ were characterized and applied to human dental pulp stem cells (hDPSCs) at concentrations of 0.5%, 1%, and 5%. We assessed cell viability, ALP activity, and mineralization through Alizarin Red staining. In addition, proteomic profiling was conducted to identify odontogenesis-related proteins. Statistical analysis was performed using the GraphPad Prism (one-way ANOVA and Tukey's post hoc test, P < 0.05). Results: WJ-MSC-derived exosomes significantly enhanced hDPSC viability and promoted differentiation in a dose-dependent manner, with maximum ALP activity and mineral deposition observed at 1% and 5% concentrations. Proteomic analysis revealed the presence of osteogenic proteins, including collagen type I alpha 1 and TGFB1. Conclusions: The findings indicate that WJ-MSC-derived exosomes significantly enhance odontogenic differentiation by promoting early and late mineralization markers, supported by the expression of osteogenic proteins. Notably, lower concentrations exhibited robust regenerative effects, highlighting their potential as cell-free therapeutics for improving healing outcomes following endodontic treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.