Evidence map›Paper›PMID 41659632›Full record

ArticlebioRxiv : the preprint server for biology2026

Coupling high-throughput protease enzymology with viral replication reveals biochemical constraints of viral fitness.

Dylan Aidlen, William Vinh Thanh Vo, Nicholas J Young, Julia Rosecrans, Anna Kurianowicz, Shih-Wei Chuo, Garrison P R Asper, Dashiell Anderson, Jacob A Posner, Duncan F Muir and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Dylan AidlenGraduate Program in Molecular and Cellular Biology, University of California, Berkeley, Berkeley, CA, USA.
William Vinh Thanh VoMaster of Translational Medicine Program, University of California, Berkeley, and University of California, San Francisco, Berkeley, San Francisco, CA, USA.
Nicholas J YoungGraduate Program in Chemistry and Chemical Biology, University of California, San Francisco, San Francisco, CA, USA.
Julia RosecransGladstone Institutes, San Francisco, CA, USA.
Anna KurianowiczGladstone Institutes, San Francisco, CA, USA.
Shih-Wei ChuoDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Garrison P R AsperDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Dashiell AndersonDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Jacob A PosnerDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Duncan F MuirGraduate Program in Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Nicholas FreitasGraduate Program in Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Melanie OttGladstone Institutes, San Francisco, CA, USA.
Charles S CraikDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Taha Y TahaGladstone Institutes, San Francisco, CA, USA.
Margaux M PinneyDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.

Funding

Targeting Viroporins and Coronavirus M ProteinU19AI171110 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI James Solomon Fraser · 2022 to 2026
$103.4M
Research Training in Chemistry and Chemical BiologyT32GM145460 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Jason E Gestwicki · 2022 to 2026
$3.1M
Leveraging Adaptive Evolution and High-Throughput Techniques to Dissect the Link Between Biochemical Function and FitnessDP5OD033413 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PINNEY, MARGAUX · 2022 to 2025
$2.0M
NIAID NIH HHS U19 AI171110NIGMS NIH HHS T32 GM145460NIH HHS DP5 OD033413
6 · The paper itself

Abstract

Proteases govern essential biological processes and are key drug targets, yet how protease sequence variation quantitatively reshapes biochemical parameters and constrains biological fitness remains poorly understood. Here, we integrate high-throughput

Identifiers

PMID41659632
PMCPMC12873778

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.