Comparison of the Microhardness of Primary Dentin With Artificially Induced Caries Following the Application of Sodium Fluoride Varnish With the Intensive Technique Versus Fluoride Therapy With Silver Diamine Fluoride.

Background and Objective: Black discoloration is a major challenge encountered when using silver diamine fluoride (SDF) to manage early childhood caries (ECC). This study aimed to assess the carious primary dentin microhardness following the application of sodium fluoride (NaF) varnish with the intensive technique versus fluoride therapy with SDF. Materials and Methods: In this in vitro study, 45 extracted relatively sound primary molars were randomly assigned to three groups: Group 1: Application of 30% SDF, Group 2: Application of Aria Dent varnish (5% NaF) three times within 10 days, and Group 3: Application of MI varnish (5% NaF + casein-phosphopeptide amorphous calcium phosphate [CPP-ACP]) three times within 10 days. After sectioning and dentin surface preparation, dentin microhardness was measured at four different time points: baseline, after demineralization, and 24 h and 45 days after the application of the respective fluoride product. The teeth were under pH-cycling throughout the entire study period. The microhardness values were compared among the groups, and at different time points using the generalized estimating equations (GEEs) model. Results: The mean microhardness at 24 h after treatment was not significantly different from that after demineralization in any group ( Conclusions: The intensive protocol of NaF varnish application three times within 10 days, especially MI varnish that contains CPP-ACP, may enhance the microhardness significantly more than SDF in the first month of use. This protocol may be able to serve as an efficient alternative to SDF.