Evidence map›Paper›PMID 41658695›Full record

ArticleCureus2026

Cloning, Expression and Purification of the Beta Subunit of Cholera Toxin Using Escherichia coli as an Expression Host and pET-24a(+) as a Cloning Vector.

Rudresh M Shoorashetty, Shwetha J Venugopal, Sneha K Chunchanur, Pooja P, Jayapriya R, Himabindu Ks, Banani Chakraborty, Ambica Rangaiah

Abstract read
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In one paragraph

Article in Cureus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rudresh M ShoorashettyDepartment of Microbiology, All India Institute of Medical Sciences (AIIMS) Bibinagar, Bibinagar, IND.
Shwetha J VenugopalDepartment of Microbiology, Bangalore Medical College and Research Institute, Bengaluru, IND.
Sneha K ChunchanurDepartment of Microbiology, Bangalore Medical College and Research Institute, Bengaluru, IND.
Pooja PDepartment of Microbiology, Employee's State Insurance Corporation Medical College (ESICMC) and Post Graduate Institute of Medical Science and Research (PGIMSR), Bengaluru, IND.
Jayapriya RDepartment of Microbiology, Bangalore Medical College and Research Institute, Bengaluru, IND.
Himabindu KsDepartment of Microbiology, Bangalore Medical College and Research Institute, Bengaluru, IND.
Banani ChakrabortyCentre for Nanoscience and Engineering, India Institute of Science, Bengaluru, IND.
Ambica RangaiahDepartment of Microbiology, Bangalore Medical College and Research Institute, Bangalore, IND.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cholera toxin has a biologically active A subunit and a binding B subunit. Being non-toxigenic, the Cholera toxin B (CTB) subunit has multiple applications in immunology and rapid diagnostics. A recombinant form of CTB (rCTB) is available for commercial use. However, the COVID-19 pandemic led to a severe global shortage in the production and distribution of commercial CTB, which posed a significant problem for researchers worldwide. Due to the non-availability of commercial rCTB post the COVID-19 pandemic, we attempted and successfully produced rCTB in a pET-based expression system in

Indexed as

cloningctxb genepet-24a(+)recombinant cholera toxin b subunitvibrio cholerae

Identifiers

PMID41658695
PMCPMC12880945

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.