ArticleFrontiers in plant science2025
Combined transcriptomic and proteomic analysis reveals the response mechanisms of alfalfa to freezing stress.
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Alfalfa ( Methods: In this study, we analyzed the physiological indices, transcriptomes and proteomes of the cold-tolerant alfalfa cultivar "Dongnong NO.1" and the cold-sensitive cultivar "Bara 218TR" at -5°C. Results: The results indicated that the levels of antioxidant enzyme and osmoregulatory substances in "Dongnong NO.1" were significantly higher than in "Bara 218TR". Additionally, the levels of malondialdehyde (MDA) and relative electrolyte leakage (REL) were found be lower in "Dongnong NO.1" than in "Bara 218TR". Further transcriptomic analysis revealed that the differentially expressed genes (DEGs) found in both alfalfa cultivars were predominantly enriched in the AP2/ERF-ERF transcription factor family and in multiple signaling pathways. Weighted gene co-expression network analysis (WGCNA) revealed that the physiological processes associated with freezing stress tolerance in the two alfalfa cultivars are closely linked to DEGs that regulate protein synthesis, calcium signaling, the inhibition of iron toxicity, and the reduction of cell wall stiffness. Proteomics analysis indicates that differentially abundant proteins (DAPs) respond to frost damage by maintaining protein stability, antioxidant defense, and metabolic regulation. Integrated transcriptomic and proteomic analyses indicate that pathways related to carbohydrate metabolism, biotic stress defense, cell wall modification, and phenylpropanoid biosynthesis are key to alfalfa's response to frost damage. Discussion: This study improves our understanding of the molecular mechanisms underlying alfalfa's freezing resistance and provides insights for the further screening and in-depth investigation of candidate genes with potential functions against freezing stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.