Evidence map›Paper›PMID 41658142›Full record

ArticleACS omega2026

A Validated Method for the Simultaneous Measurement of Tryptophan, Kynurenine, Phenylalanine, and Tyrosine by High-Performance Liquid Chromatography-Ultraviolet/Fluorescence Detection in Human Plasma and Serum.

Lucia Parráková, Cornelia A Karg, Stefanie Hofer, Pablo Monfort-Lanzas, Celina Wilgermein, Kevin Allmer, Sabine Scholl-Bürgi, Anita Siller, Harald Schennach, Simon Geisler and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lucia ParrákováInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.
Cornelia A KargInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.ORCID https://orcid.org/0009-0007-9294-586X
Stefanie HoferInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.
Pablo Monfort-LanzasInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.ORCID https://orcid.org/0000-0003-2589-3960
Celina WilgermeinDivision of Psychiatry I, Department of Psychiatry, Psychotherapy, Psychosomatics and Medical Psychology, Medical University of Innsbruck, Innsbruck 6020, Austria.
Kevin AllmerDepartment of Laboratory Medicine, Paracelsus Medical University, Salzburg 5020, Austria.
Sabine Scholl-BürgiDepartment of Paediatrics I, Medical University of Innsbruck, Innsbruck 6020, Austria.
Anita SillerCentral Institute for Blood Transfusion and Immunology, University Hospital Innsbruck, Tirol Kliniken GmbH, Innsbruck 6020, Austria.
Harald SchennachCentral Institute for Blood Transfusion and Immunology, University Hospital Innsbruck, Tirol Kliniken GmbH, Innsbruck 6020, Austria.
Simon GeislerInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.
Dietmar FuchsInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.
Thomas K FelderDepartment of Laboratory Medicine, Paracelsus Medical University, Salzburg 5020, Austria.
Johanna M GostnerInstitute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck 6020, Austria.ORCID https://orcid.org/0000-0003-1532-5177

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aromatic amino acids are precursors of neurotransmitters and immunomodulatory molecules, and their catabolism is dysregulated in various disorders associated with inflammation. This dysregulation often correlates with disease stage, symptom severity, comorbidities, quality of life, and cognitive performance, making its measurement valuable for research, diagnostics, and personalized monitoring. We developed a rapid, reliable, and cost-effective HPLC method for the simultaneous quantification of phenylalanine, tyrosine, tryptophan, and kynurenine in human serum and plasma. After protein precipitation, analytes were separated on a reversed-phase C18 column under isocratic conditions. Detection was performed based on intrinsic fluorescence for tryptophan, phenylalanine, and tyrosine and on UV absorption for kynurenine and the internal standard nitrotyrosine. The method showed linearity (R

Identifiers

PMID41658142
PMCPMC12878734

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.