ArticleFrontiers in microbiology2025
Development and partial validation of an RT-qPCR assay for the rapid detection of spring viremia of carp virus (SVCV).
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Chloroquine inhibits Spring viremia of carp virus replication by modulating autophagy and metabolic pathways.Frontiers in immunology · 2026Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spring viremia of carp (SVC), caused by spring viremia of carp virus (SVCV), is a highly contagious disease that poses a serious threat to cyprinid aquaculture and international trade, and it is listed as a notifiable disease by the World Organization for Animal Health (WOAH). Effective surveillance and control of SVCV rely on accurate and highly sensitive molecular diagnostic methods. However, several previously published RT-qPCR assays contain mismatches between primer/probe sequences and viral genomes, which may lead to false-negative results and reduced diagnostic reliability. In this study, a whole-genome comparison of 24 representative SVCV strains covering all four genotypes (SVCVa-d) was conducted, and a new primer-probe set (Cefas AR) targeting a highly conserved region of the L gene was designed. Reaction conditions were optimized, and the assay was rigorously validated in accordance with the WOAH Manual of Diagnostic Tests for Aquatic Animals. The developed RT-qPCR assay exhibited excellent analytical performance, with a limit of detection of 1.28 copies/μL, diagnostic sensitivities of 100% for cell-culture isolates and 96.6% for tissue samples, and a diagnostic specificity of 100%. In addition, the assay demonstrated strong reproducibility and consistency across nine independent laboratories. In conclusion, the WOAH-validated RT-qPCR assay developed in this study provides a highly sensitive, specific, and reliable tool for rapid screening, routine surveillance, and confirmatory diagnosis of SVCV, supporting sustainable aquaculture development and international aquatic animal health management.
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