Evidence map›Paper›PMID 41657354›Full record

ArticleJournal of neurochemistry2026

AAV-Driven miR-146a Promotes Neurite Outgrowth and Axonal Regeneration in Cortical Neurons.

V U S Matos, R A Almeida, C G Ferreira, M T R Alves, M P Braga, M C Silva, T F da Silva, P P G Guimarães, F M Soriani, P Caramelli and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

V U S MatosDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
R A AlmeidaDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
C G FerreiraDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
M T R AlvesDepartment of Physiology and Biophysics, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
M P BragaDepartment of Genetics, Ecology and Evolution, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
M C SilvaDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
T F da SilvaDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
P P G GuimarãesDepartment of Physiology and Biophysics, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
F M SorianiDepartment of Genetics, Ecology and Evolution, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
P CaramelliBehavioral and Cognitive Neurology Unit, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
M R CostaBrain Institute, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
U MichelDepartment of Neurology, University Medical Center Göttingen, Göttingen, Germany.
V T RibasDepartment of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.ORCID https://orcid.org/0000-0001-8161-786X

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 401811/2024-7Conselho Nacional de Desenvolvimento Científico e Tecnológico 406089/2024-8Conselho Nacional de Desenvolvimento Científico e Tecnológico 440498/2022-8Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-00218-17Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-01857-23Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-04985-23Fundação de Amparo à Pesquisa do Estado de Minas Gerais BPD-00704-22Fundação de Amparo à Pesquisa do Estado de Minas Gerais RED-00187-22International Society for Neurochemistry
6 · The paper itself

Abstract

Adult central nervous system (CNS) neurons exhibit limited intrinsic regenerative capacity, contributing to poor recovery after injury. MicroRNAs (miRNAs) have emerged as key regulators of many biological processes, yet their therapeutic potential in CNS repair remains incompletely understood. Here, we investigated whether adeno-associated virus (AAV) vector-mediated overexpression of miR-146a enhances neurite and axon regeneration in primary cortical neurons from Wistar rats. We found that AAV.miR-146a significantly increased neurite outgrowth, branching, and long-distance neurite regeneration following scratch injury. Using a microfluidic platform that allows us to selectively lesion axons, we further demonstrated that AAV.miR-146a robustly promotes axonal regrowth. Bioinformatic analyses revealed enrichment of miR-146a target genes involved in transcriptional regulation and synaptic function, with the inflammatory adaptor TRAF6 emerging as a key predicted target. Consistent with these predictions, AAV.miR-146a markedly reduced TRAF6 expression. Together, our results identify miR-146a as a promising therapeutic candidate for enhancing CNS axonal repair and highlight TRAF6 signaling as a potential mechanistic link to its regenerative effects.

Indexed as

AxonsCerebral CortexDependovirusMicroRNAsNerve RegenerationNeuronal OutgrowthNeuronsAnimalsCells, CulturedRatsRats, WistarMicroRNAsMIRN146a microRNA, ratAAV vectoraxon regenerationcentral nervous systemmiR‐146a

Identifiers

PMID41657354
PMCPMC12884573

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.