Evidence map›Paper›PMID 41657246›Full record

ArticleNucleic acids research2026

Condensins regulate resection-dependent DNA double-strand break repair pathways in replicated chromatin.

Mei Liu, You Wei, Lisa-Marie Weber, Emil Mladenov, Xixi Lin, Veronika Mladenova, Ramtin Omid Shafaat, Gabriel E Pantelias, Eleni Gkika, Martin Stuschke and 2 more

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In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Mei LiuDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
You WeiDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Lisa-Marie WeberDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Emil MladenovDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Xixi LinDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Veronika MladenovaDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Ramtin Omid ShafaatDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Gabriel E PanteliasInstitute of Nuclear Technology and Radiation Protection, National Centre for Scientific Research "Demokritos," Aghia Paraskevi Attikis, 15310 Athens, Greece.
Eleni GkikaRadiation Biology Laboratory, Department of Radiotherapy and Radiation Oncology, University Hospital Bonn, 53127 Bonn, Germany.
Martin StuschkeDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Aashish SoniDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.ORCID 0000-0001-9696-1006
George IliakisDivision of Experimental Radiation Biology, Department of Radiation Therapy, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.ORCID 0000-0002-1152-5342

Funding

DAAD Project 57515880German Federal Ministry for Economic Affairs BMWi-50WB1836German Research Foundation DFG-IL51.10German Research Foundation GRK1739German Research Foundation IL51.11Ministry of Education 02NUK043BMinistry of Education 02NUK054BMinistry of Education BMBF-02NUK037B
6 · The paper itself

Abstract

Condensins are key regulators of chromosome architecture and have emerging functions in DNA repair that are understudied. Here, we show that combined depletion of Condensin I and II in cell lines of normal and tumor origin selectively impairs DNA double-strand break (DSB) repair and the checkpoint response (DDR) specifically in the G2 phase of the cell cycle, with no detectable effects in G1 or S phase. Condensin knockdown increased cellular radiosensitivity and delayed in G2-phase, but not in asynchronous cells, the resolution of γH2AX and 53BP1 foci, indicating G2-specific defects in DSB repair. Mechanistically, condensin loss suppressed DNA end-resection and resection-dependent repair pathways, including homologous recombination (HR), single-strand annealing (SSA), and alternative end-joining (alt-EJ), but failed to significantly alter classical non-homologous end-joining (c-NHEJ). Reduced RAD51 and RPA70 foci formation in G2 confirmed inhibition of HR and DNA end resection. The G2 checkpoint was also compromised. Cytogenetic analysis revealed inhibition of chromosome break repair and visible chromatin decondensation, suggesting that condensins function to maintain an appropriate chromatin state for efficient DSB repair in G2-phase. These results identify for the first time condensins as G2 phase-specific regulators of genome stability by fine-tuning HR and other resection-dependent DSB repair pathways.

Indexed as

Adenosine TriphosphatasesChromatinDNA-Binding ProteinsDNA Breaks, Double-StrandedDNA RepairMultiprotein ComplexesCell Line, TumorDNA End-Joining RepairDNA ReplicationG2 PhaseHistonesHumansRecombinational DNA RepairAdenosine TriphosphatasesChromatincondensin complexesDNA-Binding ProteinsHistonesMultiprotein Complexes

Identifiers

PMID41657246
PMCPMC12884081

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.