ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences2025
[Nitrosative stress promotes the malignant biological behavior of breast cancer through the glycolytic pathway].
Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Postoperative serum oxidative stress measures in women with papillary thyroid cancer.Frontiers in endocrinology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesBreast cancer is one of the most common malignant tumors in women, and some patients face poor therapeutic efficacy and recurrence. Tumor cells are extremely dependent on the energy produced by the glycolytic pathway, which not only provides adenosine triphosphate (ATP) for rapid tumor cell proliferation, but also creates favorable conditions for tumor cell growth by regulating the acidity of the tumor microenvironment. Nitrosative stress refers to a pathophysiological state characterized by intracellular accumulation of reactive nitrogen species (RNS). Studies have found that nitrosative stress is closely associated with the occurrence and progression of cancer. This study aims to investigate the effects of nitrosative stress and tumor cell glycolysis, to provide new ideas for breast cancer treatment.
methodsPostoperative tumor tissues from breast cancer patients at the First Affiliated Hospital of Shihezi University were collected. Immunohistochemistry (IHC) staining was used to analyze the expression levels of inducible nitric oxide synthase (iNOS) and 3-nitrotyrosine (3-NT) in breast cancer tissues and adjacent normal breast tissues. Fluorescent probes were used to detect nitric oxide (NO) and reactive oxygen species (ROS) levels in normal breast cells (MCF-10A) and breast cancer cells (MDA-MB-231 and MCF-7). Western blotting was used to compare the expression levels of iNOS and 3-NT between normal and breast cancer cells. The optimal concentrations of the peroxynitrite (ONOO⁻) donor (SIN-1) and the scavenger (FeTMPyP) were determined using cell counting kit-8 (CCK-8). Glycolysis levels were measured using a glycolysis assay kit. CCK-8, Transwell, and wound-healing assays were further used to detect the proliferation, migration, and invasion abilities of MDA-MB-231 and MCF-7 cells under the effects of the two drugs. Western blotting was used to detect the expression levels of E-cadherin, N-cadherin, and vimentin, as well as glycolysis-related proteins hexokinase 2 (HK2), pyruvate kinase M2 (PKM2), and enolase 1 (ENO1).
resultsThe expression levels of iNOS and 3-NT in breast cancer tissues were significantly higher than those in normal breast tissues (
conclusionsNitrosative stress can promote the malignant biological behavior of breast cancer cells through the glycolytic pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.