ArticleThe plant pathology journal2026
A Rapid Genomic DNA Extraction Method for Direct Quantitative Polymerase Chain Reaction-Based On-Site Detection of Soil-Borne Fungal Disease.
Article in The plant pathology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Soil-borne phytopathogenic fungi cause root rot, wilt, and damping-off in crops, leading to major yield losses worldwide. Because symptoms appear only after underground infection progresses, early detection is crucial. Here, a rapid 20-min genomic DNA extraction method was developed for eight pathogens-Alternaria tenuissima, Botryosphaeria dothidea, Fusarium oxysporum, Glomerella cingulata, Phytophthora cactorum, Rosellinia necatrix, Sclerotium rolfsii, and Sclerotinia sclerotiorum. The protocol uses a cetyltrimethylammonium bromide-based buffer, steel and glass beads, brief heating (95°C, 1 min), vortexing, and sequential purification with Q-Sepharose and magnetic beads. All pathogens were detected within 30 quantitative polymerase chain reaction cycles, while soil-only controls exceeded 30 Cq. Sclerotial DNA of S. rolfsii (Cq ≈ 25) was also detected, confirming applicability for overwintering inocula. This simple and low-cost protocol enables rapid, reliable detection of multiple soil-borne fungi directly from soil, providing a practical tool for on-site disease diagnosis and management.
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