ArticleThe plant pathology journal2026
A TaqMan Real-Time PCR Assay for Early and Accurate Detection of Hypomontagnella monticulosa, an Emerging Pathogen Causing White Leaf Spot on Pachira glabra Pasq.
Article in The plant pathology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Hypomontagnella monticulosa is an emerging pathogen of Pachira glabra Pasq. causing white leaf spot, a damaging fungal disease of P. glabra in southern China. The early and proper detection and qualification is of fundamental for understanding epidemiology and developing preventive measures of this fungus. Using the second largest subunit of the RNA polymerase II gene as target, a quantitative TaqMan real-time polymerase chain reaction assay was developed for the detection and quantification of H. monticulosa in P. glabra leaves. This method could specifically recognize all tested H. monticulosa strains, while no cross-reaction was observed in closely related Hypoxylon species. Sensitivity of the assays was determined to be as low as 0.05 fg/μL (2, 300 copies/μL) of plasmid DNA, 0.5 pg/μL of mycelia genomic DNA, and 0.001% of target DNA mixed with leaf tissue DNA. The two-stage induction of H. monticulosa DNA was observed during the infection process, suggesting that this assay could be used to monitor the growth dynamics of this fungus in the whole disease process. Additionally, the assay could not only effectively detected H. monticulosa in naturally infected P. glabra trees in fields, but also accurately evaluate the differences in resistance among varieties of P. glabra. Therefore, the current study provides a rapid and accurate technology for monitoring and qualification of H. monticulosa infection in P. glabra, and will be applicable for prediction and control of the disease but also for the study of plant-H. monticulosa interaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.