ArticlePlant reproduction2026
Single and multiple buds form characteristics in Amorphophallus muelleri.
Article in Plant reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
This study investigated the physiological and molecular mechanisms underlying the formation of single-bud versus multi-bud corms in Amorphophallus muelleri foliar bulbils. Hormonal quantification revealed that multi-bud corms possessed significantly higher Cytokinin (CTK) levels and a lower Auxin/CTK ratio compared to single-bud corms. Conversely, branching inhibitors such as Brassinosteroids (BR) and Strigolactones (SLs) were significantly higher in single-bud corms. Metabolomic analysis showed a specific enrichment of fructose in multi-bud corms, suggesting high energy demand. Transcriptome sequencing identified 742 differentially expressed transcription factors and significant enrichment in hormone signaling pathways. Data reliability was validated by qRT-PCR, which confirmed the upregulation of CTK receptors (AmCRE1) and sucrose metabolism genes (AmSPS, AmINV), and the downregulation of SL signaling genes (AmD14, AmD53) in multi-bud corms. Crucially, exogenous application of 1.0 mg/L 6-BA verified the inductive role of CTK, achieving a 100% multi-bud formation rate and stabilizing the phenotype. We conclude that a high CTK background, coupled with reduced SL/BR signaling and enhanced fructose metabolism, releases apical dominance to drive multi-bud formation. These findings provide a theoretical basis and practical method for improving the propagation coefficient of konjac.
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