Evidence map›Paper›PMID 41656224›Full record

ArticleBMC plant biology2026

Effects of laser irradiation on phytochemical composition, histological anatomy, genetic diversity, and food safety of Ocimum basilicum L.

S F Desoukey, Hend S M Abdel-Aziz, Shaimaa S Shoman, Amal F Al-Shafeay, Randa S Hasan, Essam M Abdelsalam, Shaimaa R Ali

Abstract read
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

S F DesoukeyDepartment of Agriculture Botany, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Hend S M Abdel-AzizDepartment of Agriculture Botany, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Shaimaa S ShomanDepartment of Biochemistry, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Amal F Al-ShafeayAgriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, 12619, Egypt.
Randa S HasanRegional Center for Food and Feed (RCFF), Agricultural Research Center (ARC), Giza, 12619, Egypt. randasaad@arc.sci.eg.
Essam M AbdelsalamDepartment of Laser Applications in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, 12613, Egypt.
Shaimaa R AliDepartment of Laser Applications in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, 12613, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enhancing crop productivity and sustainability remains a major challenge due to the environmental impact of conventional chemical methods. Laser seed bio-stimulation offers an eco-friendly approach to improve plant growth, quality, and stress tolerance. Laser irradiation as a seed pre-treatment offers a sustainable, non-chemical approach that may contribute to improved quality and safety of medicinal and aromatic plants. This study evaluated the effects of red (650 nm) and blue (450 nm) laser irradiation for 5 and 10 min on sweet basil (Ocimum basilicum L.) seeds by analyzing vegetative growth, biochemical composition, phytohormones, and genetic variation compared to the control. Results demonstrated that 10-min laser treatment significantly enhanced plant growth, including parameters such as plant height, main stem length, number of leaves per plant, fresh and dry shoot weight, and root length, relative to the control. Laser exposure also strengthened basil leaf anatomy by thickening conducting tissues and increasing total phenolic content, antioxidant activity, pigment concentrations, phytohormone levels (GA₃ and IAA), essential oil yield, and antioxidant enzyme activity. Laser treatment modulated the essential oil composition, with variable responses among individual constituents. The impact of laser irradiation on Ocimum basilicum to induce genetic variability and enhance its physiological performance. Basil seeds were exposed to different laser type and wave length, and the resulting plants were evaluated for morphological changes and genomic polymorphism using SCoT and ISSR markers. SCoT and ISSR markers revealed that 10-min laser irradiation induced the most distinct genetic variations. SCoT primers produced 6 polymorphic bands (60%, 290–1300 bp), while ISSR primers detected 82 bands, with 22% polymorphism (150–1700 bp). Cluster analysis showed that 10-min treatments had the lowest genetic similarity, whereas 5-min treatments were more similar to the control. Combining data from both markers confirmed that red and blue laser exposure for 10 min resulted in the lowest similarity scores, while 5-min treatments exhibited the highest similarity. The present findings validate that laser irradiation can act as an eco-friendly bio-stimulation tool to enhance basil growth and biochemical composition. These results highlight its potential applications in sustainable agriculture, though further validation under open-field conditions is still required.

Indexed as

Genetic VariationLasersOcimum basilicumPhytochemicalsAntioxidantsOils, VolatilePlant Growth RegulatorsPlant LeavesSeedsAntioxidantsOils, VolatilePhytochemicalsPlant Growth RegulatorsEssential oilsLaser irradiationMolecular geneticsOcimum basilicum L.PhytochemicalsPhytohormones

Identifiers

PMID41656224
PMCPMC12931005

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.