Evidence map›Paper›PMID 41655376›Full record

ArticlePoultry science2026

Frizzled-4 promotes bone formation in chickens via activation of the canonical Wnt signaling pathway.

Xinxin Liu, Yaping Guo, Enyou Zhou, Zhiyuan An, Wei Li, Peng Cui, Weiwei Jin, Yujie Guo, Yanhua Zhang, Guoxi Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xinxin LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Yaping GuoHenan Key Laboratory of Farm Animal Breeding and Nutritional Regulation, Henan Pig Breeding Engineering Research Centre, Institute of Animal Husbandry, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Enyou ZhouCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Zhiyuan AnCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Wei LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Peng CuiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Weiwei JinCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Yujie GuoCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Yanhua ZhangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Guoxi LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Weihua TianCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Zhuanjian LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Xiangtao KangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Ruili HanCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China. Electronic address: rlhan@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Frizzled 4 (FZD4), a member of the frizzled family, is involved in various cancers and neurological disorders because of its abnormal expression. However, its regulatory role in skeletal development and bone formation, especially in livestock and poultry, remains poorly characterized. RNA sequencing (RNA-seq) analysis of bone tissue samples collected during physiological bone remodeling in chickens identified FZD4 as a differentially expressed gene. To investigate the function of FZD4 in the osteogenic differentiation of chicken bone marrow mesenchymal stem cells (BMSCs), we assessed the expression of osteogenic marker genes and canonical Wnt signaling pathway factors at the mRNA and protein levels. Additionally, we evaluated differentiation and mineralization potential via alkaline phosphatase (ALP) and alizarin red S (ARS) staining. The results demonstrated that FZD4 expression was significantly upregulated during early differentiation. Furthermore, FZD4 overexpression increased the expression of early osteogenic markers, whereas FZD4 knockdown suppressed this expression. Although FZD4 acts as a receptor for the canonical Wnt pathway, its role in promoting osteogenesis in chickens through this pathway remains unclear. Importantly, FZD4 overexpression upregulated key canonical Wnt signaling factors at both the mRNA and protein levels, whereas FZD4 knockdown had the opposite effect. These findings indicate that FZD4 participates in chicken bone formation by regulating osteogenic marker expression, likely through activation of the canonical Wnt signaling pathway. This study elucidates the specific regulatory role of FZD4 in avian skeletal development, providing novel insights and theoretical support for understanding bone development and skeletal health in poultry and livestock.

Indexed as

Avian ProteinsChickensFrizzled ReceptorsOsteogenesisWnt Signaling PathwayAnimalsMesenchymal Stem CellsAvian ProteinsFrizzled ReceptorsBMSCsBone formationCanonical Wnt pathwayChickenFZD4

Identifiers

PMID41655376
PMCPMC12907233

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.