Evidence map›Paper›PMID 41655070›Full record

ReviewJournal of separation science2026

Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications.

David S Hage, Nigar Sultana Pinky, B K Sajeeb, Md Masudur Rahman, Harshana Olupathage, Samiul Alim, Isaac Kyei, Zoe Zingler, Sanduni Heenkenda

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of separation science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

David S HageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Nigar Sultana PinkyDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
B K SajeebDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Md Masudur RahmanDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Harshana OlupathageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Samiul AlimDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Isaac KyeiDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Zoe ZinglerDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Sanduni HeenkendaDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO · 2016 to 2025
$20.8M
National Science Foundation CHE 2320239National Science Foundation CHE 2404209Nebraska Center for Biomolecular CommunicationsNIGMS NIH HHS P20 GM113126NIH HHS P20 GM113126
6 · The paper itself

Abstract

The analysis of interactions between biological agents or with surrounding chemicals is important in many areas of modern biochemical, biomedical, and environmental research. Microscale platforms based on affinity chromatography have been shown to be a powerful set of tools for these studies. This approach makes use of an immobilized binding agent as a stationary phase in a microscale platform for either direct examination of the interactions of this agent with an applied target solute or as a secondary capture agent to probe a solution-phase interaction. This review will examine the various platforms and strategies that have been used in microscale affinity chromatography, or µAC, to characterize and study biointeractions. The general principles of µAC and schemes based on this approach will be examined, along with applications of this technique. Examples of approaches that will be considered will include zonal and frontal analysis methods, as well as a variety of schemes by which µAC can be employed in kinetic studies. In each case, the theory and principles of these methods will be provided along with examples of their use in biointeraction studies.

Indexed as

Chromatography, AffinityKineticsaffinity microcolumnbinding studiesbiointeraction analysiskinetic studiesmicroscale affinity chromatography

Identifiers

PMID41655070
PMCPMC12883148

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.