Evidence map›Paper›PMID 41654633›Full record

ArticleMikrochimica acta2026

An aptamer-based "Gate-lock-key" strategy for metal-organic framework nanozyme-catalyzed colorimetric detection of multidrug-resistant bacteria.

Ruiwen Li, Hao Shen, Dandan Shi, Shaoning Yu, Guoqing Qian

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ruiwen LiDepartment of Infectious Diseases, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315211, China.
Hao ShenInstitute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China.
Dandan ShiHealth Science Center, Ningbo University, Ningbo, Zhejiang, 315211, China.
Shaoning YuInstitute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China.
Guoqing QianDepartment of Infectious Diseases, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315211, China. bill.qian@outlook.com.

Funding

Ningbo University Biomedical Innovation Institute Project H2025000422the Key Project of Zhejiang Provincial Medical and Health Science and Technology Plan WKJ-ZJ-2552
6 · The paper itself

Abstract

An aptamer-gated metal-organic framework-based biosensing platform for highly sensitive detection of multidrug-resistant bacteria (MDRB) has been developed. This biosensing platform utilizes aptamers as specific "gated" switches, which are integrated with the UiO-66-NH₂ MOFs to construct a "gated" sensing system loaded with 3,3',5,5'-tetramethylbenzidine (TMB). The entry of target bacteria induces specific dissociation of sDNA/Apt, forming an aptamer-bacteria complex on the UiO-66-NH₂ surface while releasing TMB into the solution. Subsequently, the PCN-224-Mn nanozyme catalyzes the oxidation of TMB, generating a colorimetric signal. This biosensing platform demonstrates a broad linear range for detecting New Delhi metallo-β-lactamase-1 Klebsiella pneumoniae (NDM-1 KP) and multidrug-resistant Acinetobacter baumannii (MDR-AB), achieving ultra-high sensitivity with detection limits of 5 CFU/mL and 7 CFU/mL, respectively. The practical performance of this biosensing platform was evaluated through spiked recovery experiments. Its excellent recovery demonstrates that the method maintains high reliability and stability even when applied to complex real-world samples, providing an effective technical solution for addressing challenges in microbial detection in practical scenarios.

Indexed as

Acinetobacter baumanniiAptamers, NucleotideBiosensing TechniquesColorimetryDrug Resistance, Multiple, BacterialKlebsiella pneumoniaeMetal-Organic FrameworksBenzidinesbeta-LactamasesCatalysisLimit of DetectionPhthalic Acids3,3',5,5'-tetramethylbenzidineAptamers, NucleotideBenzidinesbeta-lactamase NDM-1beta-LactamasesMetal-Organic FrameworksPhthalic AcidsUiO-66Aptamer-gated MOFsColorimetric biosensing platformMultidrug-resistant bacteriaNanozyme

Identifiers

PMID41654633

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.