ArticleMikrochimica acta2026
SERS monitoring of biothiols in serum based on thiol-alkyne click reaction.
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A novel surface-enhanced Raman scattering (SERS) sensing strategy based on thiol-yne click reactions for highly sensitive and selective detection of biothiols in serum has been developed. By combining gold-core silver-shell nanoparticles (Ag@AuNPs) with 1,4-diethynylbenzene (DTBN) and immobilizing them in an agarose hydrogel matrix, a flexible SERS sensor was constructed. The alkyne group (C ≡ C) of DTBN generated a strong characteristic signal in the Raman-silent region (2209 cm⁻1). This group underwent a click reaction with the thiol (-SH) group of biothiols under a photoinitiator, leading to a proportional attenuation of the SERS signal intensity with increasing thiol concentration. Experimental results demonstrated a linear detection range for Cys from 1 × 10⁻5 to 1 × 10⁻10 M, with a detection limit as low as 16 pM, surpassing traditional methods. Additionally, the three-dimensional network structure of the hydrogel matrix effectively shielded interference from serum components, and recoveries of 93.4%–113.3% confirmed its applicability in complex biological samples. The prepared Ag@AuNPs@DTBN hydrogel chip exhibited excellent stability and reproducibility, providing a new approach for rapid clinical monitoring of biothiols. This advancement holds promise for early warning and therapeutic evaluation of cardiovascular and neurodegenerative diseases.
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