Point-of-use colorimetric detection of Escherichia coli in food matrices with DNAzyme crosslinked hydrogels.

Hannah Mann, Akansha Prasad, Raveenaa Uthayasekaram, Kyle Jackson, Zeinab Hosseinidoust, Carlos D M Filipe, Tohid F Didar

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In one paragraph

Article in NPJ science of food, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hannah MannDepartment of Chemical Engineering, McMaster University, Hamilton, ON, Canada.

Akansha PrasadSchool of Biomedical Engineering, McMaster University, Hamilton, ON, Canada.

Raveenaa UthayasekaramDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

Kyle JacksonDepartment of Chemical Engineering, McMaster University, Hamilton, ON, Canada.

Zeinab HosseinidoustDepartment of Chemical Engineering, McMaster University, Hamilton, ON, Canada.

Carlos D M FilipeDepartment of Chemical Engineering, McMaster University, Hamilton, ON, Canada. filipec@mcmaster.ca.

Tohid F DidarSchool of Biomedical Engineering, McMaster University, Hamilton, ON, Canada. didart@mcmaster.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to the significant healthcare burden associated with foodborne illness, developing platforms suitable for the on-site detection of food pathogens is of critical importance to public health. Low-cost, equipment-free approaches are desired to allow for point-of-use contamination monitoring along the food supply chain. Here, we demonstrate the compatibility of an Escherichia coli responsive colorimetric DNAzyme-crosslinked hydrogel sensor with a wide range of food products. Sensor functionality involves an E. coli detecting DNAzyme-substrate complex that cleaves the hydrogel crosslinking in the presence of the target bacteria, resulting in a release of gold nanoparticles that is visible to the naked eye. Naked-eye detection of E. coli at concentrations of 10

Identifiers

PMID41654566

DOI10.1038/s41538-026-00745-3

PMCPMC12992606

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.