Evidence map›Paper›PMID 41654491›Full record

ArticleNature communications2026

Radical footprinting in mammalian whole blood.

Mingming Zhao, Lyle Tobin, Sandeep K Misra, Anter A Shami, Ajay Sharma, Juliette Locklar, Sayed A Mobarak, Haolin Luo, Lisa M Jones, James A Stewart and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Mingming Zhao *Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Lyle Tobin *Department of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Sandeep K MisraDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.ORCID 0000-0001-9165-3987
Anter A ShamiDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Ajay SharmaDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Juliette LocklarDepartment of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA.
Sayed A MobarakDepartment of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA.
Haolin LuoDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA.
Lisa M JonesDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0001-8825-060X
James A StewartDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Joshua S SharpDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA. jsharp@olemiss.edu.ORCID 0000-0002-0115-0276

Funding

Tracking Glycosylation State with Fluorinated Glycosides ProbesP20GM130460 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2020 to 2026
$17.9M
Molecular Structure Determination by Mass Spectrometry and Computational ModelingR01GM127267 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2018 to 2026
$3.8M
Protein Footprinting Coupled to Mass Spectrometry for the Study of Protein Higher Order Structure in Complex Model SystemsR35GM144324 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Lisa M Jones · 2022 to 2026
$3.0M
NIGMS NIH HHS P20 GM130460NIGMS NIH HHS R01 GM127267NIGMS NIH HHS R35 GM144324U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) P20GM130460U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R01GM127267U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM144324
6 · The paper itself

Abstract

Hydroxyl Radical Protein Footprinting is a powerful tool to probe protein higher-order structure, protein-protein and protein-ligand interactions. It is mostly performed in vitro, but recent advances have extended its use to live cells, nematodes, and 3D cultures. However, to the best of our knowledge, application in living mammalian tissues has not been accomplished. Here, we present the successful use of radical protein footprinting (RPF) in mammalian whole blood from wild-type and type 2 diabetes mellitus (T2DM) mice. Using persulfate photoactivated with the FOX Photolysis System, we achieve effective protein labeling without significant disruption to blood cell morphology. An optimized quenching protocol eliminates background labeling. We report oxidative modifications for the eleven most abundant proteins detected, revealing disease-associated conformational changes in multiple proteins. We validate that in-blood RPF identifies changes in protein structure resulting from complement activation and increased transferrin iron saturation in T2DM mice. These findings demonstrate the feasibility of RPF in mammalian blood and create opportunities for structural proteomics in preclinical models and clinical samples.

Indexed as

Blood ProteinsDiabetes Mellitus, Type 2Hydroxyl RadicalProtein FootprintingAnimalsComplement ActivationFemaleHumansMaleMiceMice, Inbred C57BLOxidation-ReductionPhotolysisProteomicsBlood ProteinsHydroxyl Radical

Identifiers

PMID41654491
PMCPMC12992662

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.