Evidence map›Paper›PMID 41654131›Full record

ArticleThe Journal of biological chemistry2026

GPCR-selective effects of endocytosis on cellular signaling through the cAMP/PKA cascade.

Emily E Blythe, Rita R Fagan, Mark von Zastrow

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Temporal coding expands the bandwidth of GPCR-mediated neuromodulation.bioRxiv : the preprint server for biology · 2026
    Article
  3. Advances in the Association Between theCurrent issues in molecular biology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Emily E BlytheDepartment of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, California, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address: eblythe@umn.edu.
Rita R FaganDepartment of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, California, USA.
Mark von ZastrowDepartment of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, California, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA; Quantitative Biology Institute, University of California, San Francisco, San Francisco, California, USA. Electronic address: mark.vonzastrow@ucsf.edu.

Funding

PROTEINS REGULATING ENDOCYTOSIS OF OPIOID RECEPTORSR01DA012864 · NIDA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Mark E Von Zastrow · 2000 to 2026
$7.9M
Membrane Trafficking of Opioid and Adrenergic ReceptorsR01DA010711 · NIDA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI VONZASTROW, MARK E · 2014 to 2023
$4.6M
GPR88 localization to primary cilia and its impact on striatal cAMP signalingR01MH120212 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI VONZASTROW, MARK E · 2019 to 2023
$2.0M
Mechanisms of spatiotemporal signaling by GPCRsK99GM151441 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BLYTHE, EMILY ELIZABETH · 2023 to 2024
$250k
Investigating the trafficking mechanisms and signaling consequences of dopamine receptor primary cilia localizationF32MH130096 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FAGAN, RITA REALE · 2022 to 2024
$213k
Effects of GPCR trafficking on the spatiotemporal control of signalingF32CA260118 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BLYTHE, EMILY ELIZABETH · 2021 to 2023
$189k
NCI NIH HHS F32 CA260118NIDA NIH HHS R01 DA010711NIDA NIH HHS R01 DA012864NIGMS NIH HHS K99 GM151441NIMH NIH HHS F32 MH130096NIMH NIH HHS R01 MH120212
6 · The paper itself

Abstract

Many G-protein-coupled receptors (GPCRs) use endocytosis to promote gene transcription by prolonging signaling through the Gs-coupled cAMP/cAMP-dependent PKA cascade. However, not all GPCRs efficiently internalize after agonist-induced activation, and, among those that do, considerable differences have been observed in the ability of different GPCRs to stimulate endosomal cAMP production in different cell types. We asked if endocytosis distinguishes the signaling profiles of GPCRs that are naturally coexpressed in the same cells, focusing on three Gs-coupled GPCRs endogenous to human embryonic kidney-derived (293) cells: the vasoactive intestinal peptide receptor-1 (VIPR1/VPAC1) and β2-adrenergic receptor (β

Indexed as

Cyclic AMPCyclic AMP-Dependent Protein KinasesEndocytosisReceptors, G-Protein-CoupledSignal TransductionHEK293 CellsHumansCyclic AMPCyclic AMP-Dependent Protein KinasesReceptors, G-Protein-CoupledbiosensorcAMPcell signalingDNA transcriptionendocytosisPKA

Identifiers

PMID41654131
PMCPMC13049939

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.