ArticleBMC infectious diseases2026
Association between overexpression of efflux pumps genes Rv0191 and Rv3008 and pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis: a multi-center study.
Article in BMC infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPyrazinamide is a drug that helps shorten the duration of tuberculosis treatment. Mutations in the pncA are the most common mechanism of pyrazinamide resistance in M. tuberculosis. This study aimed to investigate the association between overexpression of Efflux pump genes Rv0191 and Rv3008 and pyrazinamide resistance in multidrug-resistant M. tuberculosis in a multicenter setting.
methodsIn this study, 40 clinical isolates of pulmonary M. tuberculosis were gathered from septum specimens; confirmation of M.tuberculosis isolates was achieved by PCR and phenotypic methods using the IS6110 gene. The phenotypic microdilution broth method was evaluated for drug susceptibility testing. Frequency of pncA gene mutations associated with pyrazinamide resistance by the PCR method in M. tuberculosis isolates. The evaluation of the Rv0191 and Rv3008 efflux pump genes was performed on pyrazinamide-sensitive, pyrazinamide-resistant, and multidrug-resistant M. tuberculosis isolates.
resultsThis study showed that, in MDR, resistance to pyrazinamide was associated with single-resistant strains and a high percentage of mutations in the pncA gene. The most effective method for detecting pyrazinamide resistance is sequencing the entire pncA gene to confirm resistance, rather than the conventional techniques that focus on mutated hotspots.
conclusionsThe study found that PZA resistance was common among MDR-TB isolates, with mutations occurring in the pcnA gene. The findings also showed that drug resistance in clinical isolates of M. tuberculosis is often associated with increased expression of efflux pump genes and mutations in target genes. In cases where there are no mutations, resistance may be related to overexpression of other efflux pumps.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.