Evidence map›Paper›PMID 41650960›Full record

ArticleStem cell reports2026

DHX9 sustains hematopoietic stem cell function in cooperation with H3 acetylation.

Minhui Shi, Mengqing Gao, Huixin Luo, Chong Wang, Xueyang Hu, Yacen Xiong, Yan Chen, Xingxing Ren, Shu Zhu, Huaiping Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Minhui ShiDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China; Blood and Cell Therapy Institute, Anhui Provincial Key Laboratory of Blood Research and Applications, University of Science and Technology of China, Hefei 230027, China.
Mengqing GaoDepartment of Hematology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China; Blood and Cell Therapy Institute, Anhui Provincial Key Laboratory of Blood Research and Applications, University of Science and Technology of China, Hefei 230027, China.
Huixin LuoDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China; Blood and Cell Therapy Institute, Anhui Provincial Key Laboratory of Blood Research and Applications, University of Science and Technology of China, Hefei 230027, China.
Chong WangDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.
Xueyang HuDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.
Yacen XiongDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.
Yan ChenDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.
Xingxing RenZhejiang University Center for Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Shu ZhuInstitute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Huaiping ZhuDepartment of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China; Blood and Cell Therapy Institute, Anhui Provincial Key Laboratory of Blood Research and Applications, University of Science and Technology of China, Hefei 230027, China. Electronic address: huaipingzhu@ustc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hematopoietic stem cells (HSCs) self-renew to sustain stem cell pools and differentiate into all types of blood cells, whose properties are tightly regulated by epigenetic and transcriptional networks. Here, we identified DHX9 as a critical regulator of HSC maintenance. Dhx9 deletion caused bone marrow failure and impaired hematopoietic reconstitution in murine primary and secondary transplantation recipients due to loss of HSCs and defective self-renewal capacity. Further investigations revealed that Dhx9 deficiency led to aberrant cell cycle entry, increased apoptosis, and elevated ROS, which compromise HSC function. Mechanistically, DHX9 interacts with CBP/p300 acetyltransferase and maintains H3 acetylation at hematopoietic gene promoters to facilitate transcription activation. Inhibition of CBP/p300 disrupted their expression, whereas the enhancement of H3K27ac levels partially rescued hematopoietic defects caused by Dhx9 deficiency in both mouse models and human CD34

Indexed as

DEAD-box RNA HelicasesHematopoietic Stem CellsHistonesNeoplasm ProteinsAcetylationAnimalsApoptosisCell Self RenewalEpigenesis, GeneticHematopoiesisHumansMicep300-CBP Transcription FactorsPromoter Regions, GeneticDEAD-box RNA HelicasesDHX9 protein, humanDhx9 protein, mouseHistonesNeoplasm Proteinsp300-CBP Transcription FactorsDHX9gene transcriptionhematopoietic stem cellshistone acetylation

Identifiers

PMID41650960
PMCPMC12985375

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.