Evidence map›Paper›PMID 41648661›Full record

ArticleBiochemical engineering journal2026

Enhancing CRISPR Homology Directed Repair in IAL-PiD2 Insect Cells via Reagent Delivery Optimization and Cell Synchronization.

Bryce D Shirk, Cecilia Z Rodriguez, Marisa O Pacheco, Jasmine B McTyer, Paul D Shirk, Whitney L Stoppel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical engineering journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Bryce D ShirkJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, 32611.
Cecilia Z RodriguezChemical Engineering, University of Florida, University of Florida, Gainesville FL, 32611, USA.
Marisa O PachecoChemical Engineering, University of Florida, University of Florida, Gainesville FL, 32611, USA.
Jasmine B McTyerChemical Engineering, University of Florida, University of Florida, Gainesville FL, 32611, USA.
Paul D ShirkEntomology and Nematology, University of Florida, University of Florida, University of Florida, Gainesville FL, 32611.
Whitney L StoppelJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, 32611.

Funding

Leveraging biodiversity and utilizing genetic engineering to expand the structure and function of silk fibroin biopolymers for biomedical applicationsR35GM147041 · NIGMS · UNIVERSITY OF FLORIDA · PI Whitney L Stoppel · 2022 to 2026
$1.9M
NIGMS NIH HHS R35 GM147041
6 · The paper itself

Abstract

CRISPR/Cas9-mediated homology-directed repair (HDR) enables precise genome editing, yet its application in insect cell lines remains largely unexplored. Insect systems, already used in recombinant protein production via baculovirus expression, offer substantial potential for stable, non-viral genetic modification using HDR-based approaches. This study establishes a robust, reproducible HDR framework in

Indexed as

cell synchronizationCRISPR/Cas9genome editingHomology-Directed Repair (HDR)hydroxyureaInsect cell

Identifiers

PMID41648661
PMCPMC12872210

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.