Evidence map›Paper›PMID 41648634›Full record

ArticlebioRxiv : the preprint server for biology2026

Adapting a two-photon scanning microscope for simultaneous single-photon imaging of an infrared dopamine sensor.

Matthew Tarchick, Franklin Caval-Holme, Ben Smith, Petra Mocellin, Markita Landry, Natsumi Komatsu, Marla B Feller

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Matthew TarchickDepartment of Neuroscience and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California 94720.ORCID 0009-0007-0716-5632
Franklin Caval-HolmeDepartment of Neuroscience and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California 94720.ORCID 0000-0002-1981-5109
Ben SmithSchool of Optometry, University of California Berkeley, Berkeley, California 94720.
Petra MocellinDepartment of Neuroscience and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California 94720.
Markita LandryDepartment of Neuroscience and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California 94720.
Natsumi KomatsuDepartment of Chemical & Biomolecular Engineering, University of California Berkeley, Berkeley, California 94720.ORCID 0000-0003-0959-7088
Marla B FellerDepartment of Neuroscience and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California 94720.

Funding

UC Berkeley Vision Science COREP30EY003176 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI RICHARD H KRAMER · 1985 to 2026
$16.4M
The Function of Neural Activity in Developing RetinaR01EY013528 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Marla Feller · 2002 to 2026
$8.1M
Development of direction selectivity in the retinaR01EY019498 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI Marla Feller · 2009 to 2026
$6.6M
Transformations of photic information in melanopsin pathwaysK99EY037374 · NEI · BOSTON CHILDREN'S HOSPITAL · PI Franklin Caval-Holme · 2025 to 2026
$273k
Dissecting a microcircuit that regulates the earliest light responses in the retinaF31EY028022 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI CAVAL-HOLME, FRANKLIN · 2017 to 2019
$110k
NEI NIH HHS F31 EY028022NEI NIH HHS K99 EY037374NEI NIH HHS P30 EY003176NEI NIH HHS R01 EY013528NEI NIH HHS R01 EY019498
6 · The paper itself

Abstract

We describe a novel method for adapting a two-photon scanning microscope to enable simultaneous detection of two-photon generated visible fluorescence and single-photon generated near-infrared (nIR) fluorescence. In this configuration, nIR fluorescence is routed through a single-mode optical fiber before detection by a photomultiplier tube. This fiber coupling offers two advantages: first, the optical fiber functions as a pinhole aperture, allowing for improved optical sectioning of the nIR signal; second, it minimizes nIR background fluorescence. To validate the effectiveness of this design, we conducted two sets of experiments. First, we compare two fluorescence indicators of the neurotransmitter dopamine: the genetically encoded indicator GRAB

Identifiers

PMID41648634
PMCPMC12871380

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.