Evidence map›Paper›PMID 41648631›Full record

ArticlebioRxiv : the preprint server for biology2026

Hypatia: Comparative Isoform Profiling Across Cell Populations from Long-Read Single-Cell Transcriptomes.

Timothy Pan, Cheng-Kai Shiau, Lina Lu, Changhu Wang, Minhua Wang, Yueying He, Arvind Bhimaraj, Daniel Brat, Jason Huse, Jingyi Jessica Li and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Timothy PanDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.
Cheng-Kai ShiauDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.
Lina LuDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.
Changhu WangBiostatistics Program, Public Health Sciences Division, Fred Hutch Cancer Center, Seattle, WA, USA 98109.
Minhua WangDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.
Yueying HeDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.
Arvind BhimarajDepartment of Cardiology, Methodist DeBakey Cardiology Associates, Houston Methodist Hospital, Houston, TX, USA-77030.
Daniel BratDepartment of Pathology, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Jason HuseDepartment of Pathology and Translational Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX, USA 77030.
Jingyi Jessica LiBiostatistics Program, Public Health Sciences Division, Fred Hutch Cancer Center, Seattle, WA, USA 98109.ORCID 0000-0002-9288-5648
Ruli GaoDepartment of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 60611.

Funding

STINGing GBM: A First-in- Man Clinical Trial in Surgical Resectable Recurrent GBMP50CA221747 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Hui Zhang · 2018 to 2026
$21.4M
Single Cell Mosaic Mutation Atlas of Human OrganR35GM142539 · NIGMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI GAO, RULI · 2021 to 2025
$2.0M
NCI NIH HHS P50 CA221747NIGMS NIH HHS R35 GM142539
6 · The paper itself

Abstract

High-throughput long-read single-cell RNA-sequencing enables isoform-level study across single cells, yet methods for systematically assessing cell-to-cell variations remain limited. Here, we develop Hypatia, a comprehensive platform for dissecting isoform complexities across cell populations, devising Tsallis entropy and Cramer's V to facilitate robust comparative profiling. Hypatia revealed prominent isoform species variations and usage shifts across cell-types in glioblastoma, renal cell carcinoma, and heart, highlighting clinically relevant applications for studying isoform-derived, cell-specific functions.

Indexed as

Chi-square correctionisoform usage shiftTsallis isoform diversity

Identifiers

PMID41648631
PMCPMC12871156

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.