Evidence map›Paper›PMID 41648400›Full record

ArticlebioRxiv : the preprint server for biology2026

Modular gene tagging in

Adam Hefel, Kevin Kruse, Kaden Wall, Soren B Jorgensen, Kam Hoe Ng, Ryan Stolley, Matthew S Rich, Erik M Jorgensen

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Adam HefelSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0000-0002-2135-0152
Kevin KruseSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0000-0002-2149-1131
Kaden WallSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0009-0009-5652-339X
Soren B JorgensenSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0009-0002-8264-8451
Kam Hoe NgSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.
Ryan StolleyDepartment of Chemistry, University of Utah, Salt Lake City, Utah, USA.
Matthew S RichSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0000-0003-2353-4681
Erik M JorgensenSchool of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA.ORCID 0000-0002-2978-8028

Funding

Engineering the C. Elegans GenomeR01GM095817 · NIGMS · UNIVERSITY OF UTAH · PI JORGENSEN, ERIK M · 2011 to 2021
$2.8M
Toward a library of balanced neuronal gene knockouts in C. elegansF32GM133139 · NIGMS · UNIVERSITY OF UTAH · PI RICH, MATTHEW SAUL · 2019 to 2020
$127k
NIGMS NIH HHS F32 GM133139NIGMS NIH HHS R01 GM095817
6 · The paper itself

Abstract

Tagging a gene endogenously can identify when the gene is expressed and where the protein is localized. CRISPR is the primary tool for generating tags of endogenous genes, but it is error-prone and requires unique reagents for each gene and tag. Recombinases can insert DNA in an error-free and modular manner. Here, we tested eight recombinases for germline function in the nematode

Indexed as

B3 recombinaseC. elegans genome engineeringDregene taggingPhiC31PhIT

Identifiers

PMID41648400
PMCPMC12871313

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.