Evidence map›Paper›PMID 41648360›Full record

ArticlebioRxiv : the preprint server for biology2026

Engaging Unstabilized Alkyl Radicals with Pyridoxal Radical Biocatalysis: Enantiodivergent Synthesis of Aliphatic Non-Canonical Amino Acids.

Lei Cheng, Jasper Chen, Zhiyu Bo, Xiangyu Zhang, Peng Liu, Yang Yang

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Lei ChengDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA.ORCID 0000-0002-2473-6827
Jasper ChenDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA.
Zhiyu BoDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA.
Xiangyu ZhangDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA.
Peng LiuDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA.ORCID 0000-0002-8188-632X
Yang YangDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA.ORCID 0000-0002-4956-2034

Funding

Pyridoxal Radical Biocatalysis for the Stereoselective Production of Non-Canonical Amino AcidsR01EB036084 · NIBIB · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Yang Yang · 2025 to 2026
$1.0M
NIBIB NIH HHS R01 EB036084
6 · The paper itself

Abstract

Harnessing transient, unstabilized alkyl radical intermediates for the enantioselective construction of value-added chemical entities remains a fundamental challenge in biocatalysis. Through the repurposing and directed evolution of pyridoxal phosphate (PLP)-dependent tryptophan synthases, we advanced an open-shell enzyme platform capable of intercepting transient alkyl radicals for the efficient and enantioselective synthesis of aliphatic non-canonical amino acids. Engineering an orthogonal pair of radical PLP enzymes allowed unstabilized alkyl radicals, generated from diverse aliphatic organoboronates, to undergo dehydroxylative C(sp

Identifiers

PMID41648360
PMCPMC12871629

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.