Evidence map›Paper›PMID 41648327›Full record

ArticlebioRxiv : the preprint server for biology2026

THIK-1 channel mediates microglial glucose sensing and modulates AgRP neurons.

Qingzhuo Liu, Jonathan C Bean, Jinjing Jian, Tong Zhou, Yuxue Yang, Meixin Sun, Yongxiang Li, Kristine M Conde, Mengjie Wang, Yue Deng and 14 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Qingzhuo LiuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Jonathan C BeanUSDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.
Jinjing JianCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Tong ZhouCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yuxue YangCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Meixin SunCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yongxiang LiCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Kristine M CondeCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Mengjie WangCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yue DengCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Jiamin QiuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Fuhui WangCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Xinming LiuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yutian LiuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Jingjing ChengCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Xi WuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Lamei XueCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Chunling ChenCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Chenling MengCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yi ZhuUSDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.
Yongjie YangCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Longlong TuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Hailan LiuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.
Yong XuCenter for Molecular Psychiatry, Department of Psychiatry and Behavioral Neurosciences, University of South Florida, Tampa, FL, 33613, USA.

Funding

Neurobiology for Lac-Phe HypophagiaR01DK136479 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI YONG XU · 2023 to 2026
$2.5M
5-HT NEURONS AND MEAL REGULATIONF32DK134121 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI MCDERMOTT, KRISTINE MARIE · 2023 to 2025
$225k
NIDDK NIH HHS F32 DK134121NIDDK NIH HHS R01 DK136479
6 · The paper itself

Abstract

Microglia play essential roles in maintaining energy homeostasis, and their dysfunction contributes to metabolic disease. Although high-fat diet (HFD) exposure induces microglial activation, the underlying mechanisms remain poorly defined. Here, we identified a previously unrecognized role for THIK-1 channel in mediating glucose sensing of microglia in arcuate nucleus of the hypothalamus (ARH), during HFD-induced obesity. Pharmacological inhibition of THIK-1 channel with tetrapentylammonium (TPA) suppresses feeding and attenuates body-weight gain in diet induced obese mice. Mechanistically, inhibition of agouti-related peptide (AgRP) neurons is indispensable for TPA-induced hypophagia. Moreover, THIK-1 inhibition promotes microglial phagocytosis of perineuronal nets (PNNs), leading to reduced AgRP neuronal activity and feeding suppression. Together, these findings establish THIK-1 as a critical glucose sensor in hypothalamic microglia and uncover a microglia-dependent pathway through which overnutrition modulates AgRP neuronal activity via PNN remodeling to regulate energy balance, highlighting THIK-1 as a potential therapeutic target for treatment of diet-induced obesity.

Identifiers

PMID41648327
PMCPMC12871176

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.