Evidence map›Paper›PMID 41648320›Full record

ArticlebioRxiv : the preprint server for biology2026

Acute priming using elevated fluid viscosity recovers '

Yoseph W Dance, Alice Amitrano, Annaka M Saffron, Chanhong Min, Ladaisha T Thompson, Zhuoxu Ge, Ian M Smith, Nicolas C Macaluso, Charles Ezenwanne, Nicholas Milcik and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Yoseph W DanceDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Alice AmitranoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Annaka M SaffronDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Chanhong MinDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Ladaisha T ThompsonDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Zhuoxu GeInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Ian M SmithFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Nicolas C MacalusoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Charles EzenwanneInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Nicholas MilcikDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Abigail FennellDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Kendall A PyndellInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Kimberly M StrokaFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Jeremy D WalstonDivision of Geriatric Medicine and Gerontology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sean X SunInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.
Konstantinos KonstantopoulosDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jude M PhillipDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Funding

Technological Assessment and Solutions Core - RC4P30AG021334 · NIA · JOHNS HOPKINS UNIVERSITY · PI Jeremy D Walston · 2003 to 2026
$33.2M
Viscotaxis: Novel cell migration mechanisms regulated by microenvironmental viscosityR01CA257647 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS · 2021 to 2025
$2.3M
Exploring mechanisms of aquaporin-mediated cell migrationR35GM142838 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI STROKA, KIMBERLY · 2021 to 2025
$1.9M
Cell mechanobiology in confinement using an integration of bioengineering, materials systems and in vivo modelsR01GM142175 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS · 2021 to 2024
$1.8M
Defining a biophysical basis for cell types, cell states and cellular heterogeneity at single-cell resolutionR35GM157099 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Jude Marvin Phillip · 2025 to 2026
$762k
NCI NIH HHS R01 CA257647NIA NIH HHS P30 AG021334NIGMS NIH HHS R01 GM142175NIGMS NIH HHS R35 GM142838NIGMS NIH HHS R35 GM157099
6 · The paper itself

Abstract

Aging is a complex biological process, often characterized by increased vulnerability to disease, infection, and death. This increased vulnerability is mechanistically linked to a progressive and functional decline of the immune system. In humans, aged lymphocytes lose their capacity to effectively surveil within diverse microenvironments, decreasing their capability for clearing infections and maintaining physiological homeostasis. However, specific mechanisms by which aged lymphocytes, specifically T cells, lose this capacity to surveil remain unclear. We profiled three core characteristics of T cell surveillance at single-cell resolution, specifically migration, deformability, and sensing. While aged T cells retained their capacity for spontaneous migration, they exhibited impaired cellular deformability and deficiencies in sensing local signaling cues. To modulate this surveillance defect, we performed mechanical reprogramming using elevated fluid viscosity. Results showed that acute priming of aged T cells with elevated fluid viscosity recovered a transient

Indexed as

cell surveillanceImmune agingmechano-immunologysingle-cell motilityT cells

Identifiers

PMID41648320
PMCPMC12871368

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.