Evidence map›Paper›PMID 41648317›Full record

ArticlebioRxiv : the preprint server for biology2026

CRISPR-mediated conditional mutagenesis of

Rimma Levina, Alexander J Weitzel, Alexander Y Liu, Benjamin E Low, Pierce W Ford, Hayley Halaby, Hannah A Grunwald, Erica G Gacasan, Yasaman Moharrer, Robert L Sah and 4 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Rimma LevinaDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0001-7143-9174
Alexander J WeitzelDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-1713-158X
Alexander Y LiuDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.
Benjamin E LowThe Jackson Laboratory, Bar Harbor, Maine, USA.
Pierce W FordDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-6492-593X
Hayley HalabyDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.
Hannah A GrunwaldDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-0553-906X
Erica G GacasanShu Chien-Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-3145-7615
Yasaman MoharrerDepartment of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID 0009-0008-8428-3461
Robert L SahShu Chien-Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-5493-9502
Eric J BennettDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0002-1201-3314
Joel D BoerckelDepartment of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID 0000-0003-3126-3025
Michael V WilesThe Jackson Laboratory, Bar Harbor, Maine, USA.ORCID 0000-0002-3620-8386
Kimberly L CooperDepartment of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0001-5892-8838

Funding

VirologyP30AI036214 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUSAN JANET LITTLE · 1994 to 2026
$78.4M
Pathways in Biological Sciences Training ProgramT32GM133351 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Matthew Daugherty, Randolph Y. Hampton · 2020 to 2026
$9.9M
Development of approaches to apply CRISPR/Cas9-mediated gene conversion to model complex genetic traits in miceR01GM148640 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kimberly Lynn Cooper · 2023 to 2026
$2.2M
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
Engineering and validation of two conditional multi-gene mouse models of skeletal developmentR21AR077799 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI COOPER, KIMBERLY LYNN · 2020 to 2021
$354k
NIAID NIH HHS P30 AI036214NIAMS NIH HHS R21 AR077799NIGMS NIH HHS R01 GM148640NIGMS NIH HHS T32 GM133351NIH HHS S10 OD026929
6 · The paper itself

Abstract

The BMP/GDF branch of TGF-β signaling regulates diverse aspects of skeletal biology, from skeletal development to maintenance and repair. However, the complexity, redundancy, and pleiotropy of BMP/GDF signaling have hamstrung a genetic dissection of its activities in different cell types over time. Here, we tested the feasibility of a three-transgene system using CRISPR/Cas9 to conditionally mutate six target sites, two each in the receptor-mediated

Identifiers

PMID41648317
PMCPMC12871695

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.