Evidence map›Paper›PMID 41648288›Full record

ArticlebioRxiv : the preprint server for biology2026

Fusion gene discovery in single cells from high throughput long read single cell transcriptomes.

Cheng-Kai Shiau, Hsiao-Yun Lin, Timothy Pan, Xiaodong Lu, Minhua Wang, Yueying He, Lina Lu, Jindan Yu, Ruli Gao

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cheng-Kai ShiauDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Hsiao-Yun LinDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Timothy PanDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Xiaodong LuDepartment of Urology, Emory University - School of Medicine, Atlanta, GA, USA 30322.
Minhua WangDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Yueying HeDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Lina LuDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.
Jindan YuDepartment of Urology, Emory University - School of Medicine, Atlanta, GA, USA 30322.
Ruli GaoDepartment of Biochemistry and Molecular Genetics, Northwestern University - Feinberg School of Medicine, Chicago, IL, USA 60611.ORCID 0000-0002-1137-4564

Funding

Single Cell Mosaic Mutation Atlas of Human OrganR35GM142539 · NIGMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI GAO, RULI · 2021 to 2025
$2.0M
Defining cellular mechanisms of chronic graft failure in transplanted hearts with single cell multi-omicsR01HL160552 · NHLBI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI GAO, RULI · 2022 to 2025
$1.7M
NHLBI NIH HHS R01 HL160552NIGMS NIH HHS R35 GM142539
6 · The paper itself

Abstract

High throughput long-read single cell RNA sequencing allows for fusion gene discovery in thousands of cells in parallel, which is however constrained by the lack of robust computational tools. Here, we develop LongFUSE that employs the XOR logic operation and stringent filtering criteria for the accurate detection of cell-specific fusion genes and their splicing isoforms. LongFUSE outperforms existing tools in both simulation and real data, demonstrating high accuracy and robustness.

Identifiers

PMID41648288
PMCPMC12871245

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.