Evidence map›Paper›PMID 41648250›Full record

ArticlebioRxiv : the preprint server for biology2026

Efficient transgene-free multiplexed germline editing via viral delivery of an engineered TnpB.

Trevor Weiss, Maris Kamalu, Honglue Shi, Gabriel Wirnowski, Alice Ingelsson, Jasmine Amerasekera, Kamakshi Vohra, Marena I Trinidad, Zheng Li, Emily Freitas and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Trevor WeissDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Maris KamaluDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Honglue ShiInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0003-3847-1652
Gabriel WirnowskiDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Alice IngelssonDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Jasmine AmerasekeraDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Kamakshi VohraInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Marena I TrinidadInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Zheng LiDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Emily FreitasDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Noah SteinmetzDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Charlie AmbroseDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Kerry ChenDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Jennifer A DoudnaInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0001-9161-999X
Steven E JacobsenDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.

Funding

The dynamics, functions and evolution of a miniature RNA-guided enzymeK99GM160778 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Honglue Shi · 2025 to 2026
$240k
NIGMS NIH HHS K99 GM160778
6 · The paper itself

Abstract

Virus-induced genome editing (VIGE) using compact RNA-guided endonucleases is a transformational new approach in plant biotechnology, enabling tissue-culture-independent and transgene-free genome editing (Hu et al. 2025; Liu et al. 2025; Weiss et al. 2025). We recently established a VIGE approach for heritable editing at single loci in

Identifiers

PMID41648250
PMCPMC12871731

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.