Evidence map›Paper›PMID 41648130›Full record

ArticlebioRxiv : the preprint server for biology2026

Monitoring Gene Expression in Retina with synthetic serum markers.

Jiaxiong Lu, Sangsin Lee, Meng Wang, Karen Zheng, Soo Hwan Oh, Jocelyn Lee, Sujung Soh, Yexuan Cao, Xuan Bao, Yifan Huang and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jiaxiong LuGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Sangsin LeeDepartment of Bioengineering, Rice University, Houston, TX, USA. 77030.ORCID 0000-0002-1306-2206
Meng WangGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Karen ZhengDepartment of Bioengineering, Rice University, Houston, TX, USA. 77030.
Soo Hwan OhDepartment of Bioengineering, Rice University, Houston, TX, USA. 77030.
Jocelyn LeeDepartment of Bioengineering, Rice University, Houston, TX, USA. 77030.
Sujung SohGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Yexuan CaoGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Xuan BaoGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Yifan HuangGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.
Shirin NouraeinRice Neuroengineering Initiative, Rice University, Houston, TX, USA. 77030.ORCID 0000-0003-3423-1002
Zigang WuRice Neuroengineering Initiative, Rice University, Houston, TX, USA. 77030.
Jerzy O SzablowskiDepartment of Bioengineering, Rice University, Houston, TX, USA. 77030.ORCID 0000-0001-7851-5408
Rui ChenGavin Herbert Eye Institute-Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA USA. 92617.ORCID 0000-0002-4387-9735

Funding

GENETICS OF EARLY ONSET RETINAL DISEASESR01EY018571 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI RUI CHEN · 2008 to 2026
$7.2M
Molecular Basis of Human Visual System DisordersR01EY022356 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI RUI CHEN · 2012 to 2026
$6.1M
Monitoring neuronal activity with a blood test - Released Markers of Activity (RMA)DP2EB035905 · NIBIB · RICE UNIVERSITY · PI Jerzy Olgierd Szablowski · 2023 to 2026
$2.3M
Molecular Mechanisms of Human Retinal DiseaseR01EY020540 · NEI · BAYLOR COLLEGE OF MEDICINE · PI CHEN, RUI, MARDON, GRAEME · 2011 to 2017
$2.3M
Noninvasive site-specific measurement of gene expression in deep tissues with secreted reportersR21EB033059 · NIBIB · RICE UNIVERSITY · PI SZABLOWSKI, JERZY OLGIERD · 2022 to 2024
$586k
NEI NIH HHS R01 EY018571NEI NIH HHS R01 EY020540NEI NIH HHS R01 EY022356NIBIB NIH HHS DP2 EB035905NIBIB NIH HHS R21 EB033059
6 · The paper itself

Abstract

Gene expression underlies retinal development, function, and pathogenesis. However, monitoring retinal gene expression in vivo is challenging. In this study, we apply synthetic serum markers, called Released Markers of Activity, or RMAs, to quantify gene expression in an intact retina through a simple blood test. We show that RMAs can quantify transduction of multiple retinal cell-types and monitor cell implantation or cell loss. We found RMAs are sensitive enough to measure transduction of rare cell populations, such as retinal ganglion cells and could detect transplantation of as few as 100 stem cells. Expression of RMAs showed no evidence of cell loss or immune activation in the retina. Overall, these findings demonstrate that RMAs are highly sensitive, tissue non-destructive gene expression reporters that can continuously track gene delivery and cell survival in intact retina through a simple blood test.

Identifiers

PMID41648130
PMCPMC12871320

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.