Evidence map›Paper›PMID 41648110›Full record

ArticlebioRxiv : the preprint server for biology2026

Bright calcium-modulated bioluminescent indicators for activity imaging and red photon-assisted synaptic transmission.

Yufeng Zhao, Montserrat Porta-de-la-Riva, Sungmoo Lee, Yan Wu, Mark Klein, Mary P Hall, Yichi Su, Lance P Encell, Thomas A Kirkland, Michael Krieg and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yufeng ZhaoDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-1788-7969
Montserrat Porta-de-la-RivaICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, Castelldefels (Barcelona) 08860, Spain.
Sungmoo LeeDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Yan WuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Mark KleinPromega Corporation, Madison, WI 53711, USA.
Mary P HallPromega Corporation, Madison, WI 53711, USA.
Yichi SuDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Lance P EncellPromega Corporation, Madison, WI 53711, USA.
Thomas A KirklandPromega Corporation, Madison, WI 53711, USA.
Michael KriegICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, Castelldefels (Barcelona) 08860, Spain.ORCID 0000-0003-0501-5036
Michael Z LinDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-0492-1961

Funding

The power of positivity: a novel class of voltage indicators for high-fidelity brain activity imagingR01NS123681 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2021 to 2021
$3.6M
Noninvasive bioluminescent imaging of neuronal activity in freely behaving animalsR21DA048252 · NIDA · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2019 to 2020
$435k
Bioluminescent indicators for noninvasive imaging of acetylcholine releaseR21NS122055 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2021 to 2021
$434k
NIDA NIH HHS R21 DA048252NINDS NIH HHS R01 NS123681NINDS NIH HHS R21 NS122055
6 · The paper itself

Abstract

Bioluminescent calcium sensors have unique uses in neuroscience and neuroengineering, enabling noninvasive imaging of neuronal activity and contactless activation of opsin-expressing neurons. However, the speed, range, and robustness of non-invasive imaging and rewiring or neuronal activity are limited by the radiance and dynamic range of existing bioluminescent calcium sensors. Here, we report the stepwise engineering of improved cyan-excitable red fluorescent proteins (mCyRFP4 and dCyRFP4), an improved red bioluminescent protein based on NanoLuc and CyRFP4 (Antares3), and an improved bioluminescent calcium sensor based on Antares3 (CaMBI3). Antares3 is 3-fold brighter than its predecessor, while CaMBI3 responds with an overall dynamic range of 24-fold, enabling high-sensitivity detection of calcium dynamics in muscle and neuronal tissues

Identifiers

PMID41648110
PMCPMC12871638

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.